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Literature summary extracted from

  • Bjoerkelid, C.; Bergfors, T.; Raichurkar, A.K.; Mukherjee, K.; Malolanarasimhan, K.; Bandodkar, B.; Jones, T.A.
    Structural and biochemical characterization of compounds inhibiting Mycobacterium tuberculosis pantothenate kinase (2013), J. Biol. Chem., 288, 18260-18270.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 expressed in Escherichia coli BL21-AI cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.33 sitting drop vapor diffusion method, using 14.4% (w/v) PEG 8000, 20% (v/v) glycerol, 0.08 m sodium cacodylate, pH 6.5, and 0.16 m magnesium acetate Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 2,6-difluoro-N-[1-(5-[[2-(4-fluorophenoxy)ethyl]sulfanyl]-4-methyl-4H-1,2,4-triazol-3-yl)ethyl]benzamide competitive inhibition Mycobacterium tuberculosis
2.7.1.33 2-chloro-N-[1-(5-[[2-(4-fluorophenoxy)ethyl]sulfanyl]-4-methyl-4H-1,2,4-triazol-3-yl)ethyl]benzamide competitive inhibition Mycobacterium tuberculosis
2.7.1.33 2-[(4'-cyano-2-[[4-(pyridin-2-yl)piperazin-1-yl]methyl][1,1'-biphenyl]-4-yl)oxy]-1-oxoethan-1-aminide non-competitive inhibition Mycobacterium tuberculosis
2.7.1.33 N-(1-[5-[(4-fluorobenzyl)sulfanyl]-4-methyl-4H-1,2,4-triazol-3-yl]ethyl)-2-(trifluoromethyl)benzamide competitive inhibition Mycobacterium tuberculosis
2.7.1.33 N-[1-(5-[[2-(4-fluorophenoxy)ethyl]sulfanyl]-4-methyl-4H-1,2,4-triazol-3-yl)ethyl]-2-(trifluoromethyl)benzamide competitive inhibition Mycobacterium tuberculosis
2.7.1.33 N-[1-(5-[[2-(4-fluorophenoxy)ethyl]sulfanyl]-4-[(4-fluorophenyl)methyl]-4H-1,2,4-triazol-3-yl)ethyl]-2-(trifluoromethyl)benzamide competitive inhibition Mycobacterium tuberculosis
2.7.1.33 [(4'-cyano-2-[[4-(pyridin-2-yl)piperazin-1-yl]methyl][1,1'-biphenyl]-4-yl)oxy]acetic acid non-competitive inhibition Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate Mycobacterium tuberculosis
-
 ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Mycobacterium tuberculosis P9WPA7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33 nickel-Sepharose column chromatography and Superdex 75 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Mycobacterium tuberculosis ADP + (R)-4'-phosphopantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.33 homodimer
-
 Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 CoaA
-
 Mycobacterium tuberculosis
2.7.1.33 PanK
-
 Mycobacterium tuberculosis
2.7.1.33 Rv1092c
-
 Mycobacterium tuberculosis