EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.1 | gene bbc, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression from vector mammalian expression vector pcDNA3.1/V5-His-TOPO in HeLa cells and in Drosophila S2 cells | Drosophila melanogaster |
2.7.8.2 | gene CG7149, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression from vector mammalian expression vector pcDNA3.1/V5-His-TOPO in HeLa cells and in Drosophila S2 cells | Drosophila melanogaster |
2.7.8.B14 | gene CG4585, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression from vector mammalian expression vector pcDNA3.1/V5-His-TOPO in HeLa cells and in Drosophila S2 cells | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.8.1 | endoplasmic reticulum | exclusive | Drosophila melanogaster | 5783 | - |
2.7.8.2 | Golgi apparatus | - |
Drosophila melanogaster | 5794 | - |
2.7.8.B14 | Golgi membrane | the enzyme resides in the Golgi complex with its active site facing the lumen | Drosophila melanogaster | 139 | - |
2.7.8.B14 | plasma membrane | at high expression level | Drosophila melanogaster | 5886 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.B14 | Mn2+ | the enzyme activity is strictly dependent on the presence of Mn2+ ions | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.1 | CDP-ethanolamine + 1,2-diacyl-sn-glycerol | Drosophila melanogaster | - |
CMP + a phosphatidylethanolamine | - |
r | |
2.7.8.2 | CDP-choline + 1,2-diacyl-sn-glycerol | Drosophila melanogaster | - |
CMP + a phosphatidylcholine | - |
r | |
2.7.8.2 | CDP-ethanolamine + 1,2-diacyl-sn-glycerol | Drosophila melanogaster | - |
CMP + a phosphatidylethanolamine | - |
r | |
2.7.8.2 | additional information | Drosophila melanogaster | the enzyme has dual specificity for both CDP-choline and CDP-ethanolamine, EC 2.7.8.1 | ? | - |
? | |
2.7.8.B14 | a ceramide + a phosphatidylethanolamine | Drosophila melanogaster | - |
a ceramide phosphoethanolamine + a 1,2-diacyl-sn-glycerol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.1 | Drosophila melanogaster | A1Z9E0 | - |
- |
2.7.8.2 | Drosophila melanogaster | Q8T0S3 | - |
- |
2.7.8.B14 | Drosophila melanogaster | O77475 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.1 | CDP-ethanolamine + 1,2-diacyl-sn-glycerol | - |
Drosophila melanogaster | CMP + a phosphatidylethanolamine | - |
r | |
2.7.8.2 | CDP-choline + 1,2-diacyl-sn-glycerol | - |
Drosophila melanogaster | CMP + a phosphatidylcholine | - |
r | |
2.7.8.2 | CDP-ethanolamine + 1,2-diacyl-sn-glycerol | - |
Drosophila melanogaster | CMP + a phosphatidylethanolamine | - |
r | |
2.7.8.2 | additional information | the enzyme has dual specificity for both CDP-choline and CDP-ethanolamine, EC 2.7.8.1 | Drosophila melanogaster | ? | - |
? | |
2.7.8.B14 | a ceramide + a phosphatidylethanolamine | - |
Drosophila melanogaster | a ceramide phosphoethanolamine + a 1,2-diacyl-sn-glycerol | - |
? | |
2.7.8.B14 | a ceramide + a phosphatidylethanolamine | assay substrate is C6-NBD-ceramide | Drosophila melanogaster | a ceramide phosphoethanolamine + a 1,2-diacyl-sn-glycerol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.1 | Bb in a boxcar, isoform B | UniProt | Drosophila melanogaster |
2.7.8.1 | bbc | - |
Drosophila melanogaster |
2.7.8.1 | CEPT | - |
Drosophila melanogaster |
2.7.8.1 | CG6016 | locus name | Drosophila melanogaster |
2.7.8.1 | dCCS2 | - |
Drosophila melanogaster |
2.7.8.1 | ethanolamine phosphotransferase | - |
Drosophila melanogaster |
2.7.8.2 | CEPT | - |
Drosophila melanogaster |
2.7.8.2 | CG7149 | - |
Drosophila melanogaster |
2.7.8.2 | CG7149, isoform A | UniProt | Drosophila melanogaster |
2.7.8.2 | dCCS3 | - |
Drosophila melanogaster |
2.7.8.2 | diacylglycerol cholinephosphotransferase | - |
Drosophila melanogaster |
2.7.8.B14 | CCS | - |
Drosophila melanogaster |
2.7.8.B14 | CDP-Eth:ceramide ethanolamine phosphotransferase | - |
Drosophila melanogaster |
2.7.8.B14 | CDP-ethanolamine:ceramide ethanolamine phosphotransferase | - |
Drosophila melanogaster |
2.7.8.B14 | ceramide ethanolamine phosphotransferase | - |
Drosophila melanogaster |
2.7.8.B14 | CG4585 | - |
Drosophila melanogaster |
2.7.8.B14 | CPE synthase | - |
Drosophila melanogaster |
2.7.8.B14 | cpeS | - |
Drosophila melanogaster |
2.7.8.B14 | dCCS4 | - |
Drosophila melanogaster |
2.7.8.B14 | monofunctional CPE synthase | - |
Drosophila melanogaster |
2.7.8.B14 | PE:ceramide ethanolamine phosphotransferase | - |
Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.8.B14 | 27 | - |
assay at | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.8.B14 | 7 | - |
assay at | Drosophila melanogaster |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.8.1 | evolution | the enzyme belongs to the CEPT subfamily | Drosophila melanogaster |
2.7.8.2 | evolution | the enzyme belongs to the CEPT subfamily | Drosophila melanogaster |
2.7.8.B14 | evolution | insect-specific CPE synthase belongs to a novel branch of CDP-alcohol phosphotransferases with unique membrane topology. CPE synthase constitutes a new branch within the CDP-alcohol phosphotransferase superfamily with homologues in Arthropoda (insects, spiders, mites, scorpions), Cnidaria (Hydra, sea anemones), and Mollusca (oysters) but not in most other animal phyla. The enzyme resides in the Golgi complex with its active site facing the lumen, contrary to the membrane topology of other CDPalcohol phosphotransferases | Drosophila melanogaster |
2.7.8.B14 | malfunction | depletion of dCCS4 caused a major (about 60%) reduction in CPES activity. When incubated with CDP-[14C]Eth in the presence of Mn2+ ions, lysates of dCCS4-depleted cells synthesize only a minor (about 25%) fraction of the radiolabeled CPE formed in lysates of control (dsGFP-treated) cells. In addition, loss of dCCS4 causes a substantial drop in de novo synthesis of CPE, accompanied by a defect in cell growth | Drosophila melanogaster |
2.7.8.B14 | metabolism | ceramide phosphoethanolamine (CPE) is the principal membrane sphingolipid. The corresponding CPE synthase shares mechanistic features with enzymes mediating phospholipid biosynthesis via the Kennedy pathway, e.g. EC 2.7.8.2. Drosophila lacks the phosphocholine-containing sphingomyelin (SM) found in mammalian membranes and instead synthesizes ceramide phosphoethanolamine (CPE). SMS2, EC 2.7.8.27, is a bifunctional enzyme that produces both SM and CPE, SMS2 likely accounts for the plasma membrane-resident CPE synthase activity | Drosophila melanogaster |
2.7.8.B14 | physiological function | CDP-ethanolamine:ceramide ethanolamine phosphotransferase is the enzyme responsible for bulk production of ceramide phosphoethanolamine (CPE) in Drosophila. The smaller crosssectional area of the phosphoethanolamine headgroup in CPE allows a closer contact between these molecules in comparison with SM, promoting membrane viscosity. Contrary to sphingomyelin, CPE does not interact favorably with cholesterol and fails to form sterol-rich domains in model bilayers. The addition of CDP-Eth to lysates of HeLa cells expressing dCCS4 caused a dramatic increase in NBD-CPE formation. Drosophila S2 cells require dCCS4 for CDP-Eth-dependent CPE production and growth | Drosophila melanogaster |