Literature summary extracted from
Ouchane, S.; Steunou, A.S.; Picaud, M.; Astier, C.
Aerobic and anaerobic Mg-protoporphyrin monomethyl ester cyclases in purple bacteria: a strategy adopted to bypass the repressive oxygen control system (2004), J. Biol. Chem., 279, 6385-6394.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.21.98.3 |
expression in Escherichia coli |
Rubrivivax gelatinosus |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.21.98.3 |
43000 |
- |
- |
Rubrivivax gelatinosus |
1.21.98.3 |
63000 |
- |
- |
Rubrivivax gelatinosus |
1.21.98.3 |
86000 |
- |
SDS-PAGE of truncated protein of 384 residues |
Rubrivivax gelatinosus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.21.98.3 |
Rubrivivax gelatinosus |
Q7X2C7 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.21.98.3 |
recombinant protein, preparation is yellow-brown in color. Only a small fraction can be solubilized with urea (6 M), and most of the iron-sulfur cluster is degraded upon solubilization of inclusion bodies |
Rubrivivax gelatinosus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.21.98.3 |
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O |
- |
Rubrivivax gelatinosus |
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine |
overall reaction |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.21.98.3 |
dimer |
2 * 63000, calculated, 2 * 43000, SDS-PAGE of truncated protein of 384 residues |
Rubrivivax gelatinosus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.21.98.3 |
BChE |
- |
Rubrivivax gelatinosus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.21.98.3 |
[4Fe-4S]-center |
BchE sequence displays the conserved CXXXCXXC sequence essential for 4Fe-4S cluster formation and an active 4Fe-4S cluster is present in the protein. Isolated protein is yellow-brown in colour with an absorption peak around 410 nm |
Rubrivivax gelatinosus |
|
Expression
EC Number |
Organism |
Comment |
Expression |
---|
1.21.98.3 |
Rubrivivax gelatinosus |
expression of the BchE gene is oxygen-independent |
additional information |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.21.98.3 |
physiological function |
a BchE mutant is photosynthesis-deficient, produces bacteriochlorophyll only under high oxygenation and accumulates Mg-protoporphyrin monomethyl ester under low oxygenation and anaerobiosis. A double knockout mutant lacking both Bche and aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase AcsF is devoid of photosystem and accumulates Mg-protoporphyrin monomethyl ester under both conditions indicating the involvement of the two enzymes at the same step of the biosynthesis pathway. AcsF acts strictly under high oxygenation conditions, whereas BchE is involved when the oxygen tension drops |
Rubrivivax gelatinosus |