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Literature summary extracted from

  • Terada, T.; Kitazume, S.; Kitajima, K.; Inoue, S.; Ito, F.; Troy, F.; Inoue, Y.
    Synthesis of CMP-deaminoneuraminic acid (CMP-KDN) using the CTP:CMP-3-deoxynonulosonate cytidylyltransferase from rainbow trout testis: Identification and characterization of a CMP-KDN synthetase (1993), J. Biol. Chem., 268, 2640-2648.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.92 1.7
-
 CTP cosubstrate N-glycolylneuraminic acid, pH 9, 25°C Oncorhynchus mykiss
2.7.7.92 2.6
-
 CTP cosubstrate 3-deoxy-D-glycero-D-galacto-nononate, pH 9, 25°C Oncorhynchus mykiss
2.7.7.92 2.7
-
 CTP cosubstrate N-acetylneuraminic acid, pH 9, 25°C Oncorhynchus mykiss

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.92 Mg2+ or Mn 2+, required. 25 mg2+ stimulates reaction with 3-deoxy-D-glycero-D-galacto-nononate more than 10fold Oncorhynchus mykiss

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.92 260000
-
 gel filtration Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.92 CTP + 3-deoxy-D-glycero-D-galacto-nononate Oncorhynchus mykiss preferred substrate diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
2.7.7.92 CTP + N-acetylneuraminic acid Oncorhynchus mykiss
-
 diphosphate + CMP-N-acetylneuraminic acid
-
 ?
2.7.7.92 CTP + N-glycolylneuraminic acid Oncorhynchus mykiss
-
 diphosphate + CMP-N-glycolylneuraminic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.92 Oncorhynchus mykiss Q90WG6 cf. EC 2.7.7.43
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.92 sperm expression of CMP-KDN synthetase is temporally correlated with development and parallels the developmental expression of (KDN)GM3 in sperm Oncorhynchus mykiss
-
2.7.7.92 testis
-
 Oncorhynchus mykiss
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.92 CTP + 3-deoxy-D-glycero-D-galacto-nononate preferred substrate Oncorhynchus mykiss diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
-
 ?
2.7.7.92 CTP + N-acetylneuraminic acid
-
 Oncorhynchus mykiss diphosphate + CMP-N-acetylneuraminic acid
-
 ?
2.7.7.92 CTP + N-glycolylneuraminic acid
-
 Oncorhynchus mykiss diphosphate + CMP-N-glycolylneuraminic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.92 CMP-KDN synthetase
-
 Oncorhynchus mykiss

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.92 25
-
-
 Oncorhynchus mykiss

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.92 9 10
-
 Oncorhynchus mykiss