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Literature summary extracted from

  • Zuniga-Ripa, A.; Barbier, T.; Conde-Alvarez, R.; Martinez-Gomez, E.; Palacios-Chaves, L.; Gil-Ramirez, Y.; Grillo, M.J.; Letesson, J.J.; Iriarte, M.; Moriyon, I.
    Brucella abortus depends on pyruvate phosphate dikinase and malic enzyme but not on Fbp and GlpX fructose-1,6-bisphosphatases for full virulence in laboratory models (2014), J. Bacteriol., 196, 3045-3057.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.1 gene ppdK, expression analysis Brucella abortus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.1 additional information construction of an enzyme deletion mutant DELTAppdK from strain 2308, and of a double deletion mutant DELTApckADELTAppdK, also lacking phosphoenolpyruvate carboxykinase, both show a reduced growth phenotype, overview. But albeit impaired in growth, the DELTAppdK is still able to reach the replicative intracellular niche, but BABDELTAppdK fails to reach the chronic steady phase typical of virulent brucellae Brucella abortus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.1 Mg2+ required Brucella abortus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate Brucella abortus
-
 AMP + phosphoenolpyruvate + diphosphate
-
 r
2.7.9.1 ATP + pyruvate + phosphate Brucella abortus 2308
-
 AMP + phosphoenolpyruvate + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Brucella abortus Q2YMM9
-
-
2.7.9.1 Brucella abortus 2308 Q2YMM9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.9.1 macrophage
-
 Brucella abortus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate
-
 Brucella abortus AMP + phosphoenolpyruvate + diphosphate
-
 r
2.7.9.1 ATP + pyruvate + phosphate
-
 Brucella abortus 2308 AMP + phosphoenolpyruvate + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.9.1 More putative Brucella abortus 2308 PpdK, encoded by BAB1_0525, represents a complete protein of 887 amino acids with the PEP-binding (amino acids 19 to 376) and the TIM barrel (amino acids 530 to 883) domains characteristic of PEP-utilizing enzymes Brucella abortus

Synonyms

EC Number Synonyms Comment Organism
2.7.9.1 BAB1_0525 locus name Brucella abortus
2.7.9.1 PPDK
-
 Brucella abortus
2.7.9.1 pyruvate phosphate dikinase
-
 Brucella abortus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.1 AMP
-
 Brucella abortus
2.7.9.1 ATP
-
 Brucella abortus

General Information

EC Number General Information Comment Organism
2.7.9.1 malfunction in gluconeogenic but not in rich media, growth of enzyme-deficient mutant DELTAppdK is severely impaired. In RAW 264.7 macrophages, the DELTAppdK mutant shows reduced multiplication, and studies with the DELTAppdK mutant confirm that it reaches the replicative niche. The mutant is attenuated in mice being cleared by week 10 Brucella abortus
2.7.9.1 metabolism the enzyme plays an important role in the central C metabolism between gluconeogenesis, glycolysis, and TCA cycle. Dysfunction of Brucella abortus fbp, glpX, ppdK, and mae but not of pckA or aceA homologues affects growth on gluconeogenic substrates in vitro Brucella abortus