Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kertesz, M.A.; Schmidt-Larbig, K.; Wueest, T.
    A novel reduced flavin mononucleotide-dependent methanesulfonate sulfonatase encoded by the sulfur-regulated msu operon of Pseudomonas aeruginosa (1999), J. Bacteriol., 181, 1464-1473.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.34 expression in Escherichia coli Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.34 41600
-
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.34 Pseudomonas aeruginosa Q9I1C2
-
-
1.14.14.34 Pseudomonas aeruginosa DSM 22644 Q9I1C2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.34 ethanesulfonate + FMNH2 + O2 27% of the activity with methanesulfonate Pseudomonas aeruginosa acetaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 ethanesulfonate + FMNH2 + O2 27% of the activity with methanesulfonate Pseudomonas aeruginosa DSM 22644 acetaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 isethionate + FMNH2 + O2 19% of the activity with methanesulfonate Pseudomonas aeruginosa hydroxyacetaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 isethionate + FMNH2 + O2 19% of the activity with methanesulfonate Pseudomonas aeruginosa DSM 22644 hydroxyacetaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 methanesulfonate + FMNH2 + O2
-
 Pseudomonas aeruginosa formaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 methanesulfonate + FMNH2 + O2
-
 Pseudomonas aeruginosa DSM 22644 formaldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.34 morpholinepropanesulfonic acid + FMNH2 + O2 17% of the activity with methanesulfonate Pseudomonas aeruginosa 3-(morpholin-4-yl)propanal + FMN + sulfite + H2O
-
 ?
1.14.14.34 morpholinepropanesulfonic acid + FMNH2 + O2 17% of the activity with methanesulfonate Pseudomonas aeruginosa DSM 22644 3-(morpholin-4-yl)propanal + FMN + sulfite + H2O
-
 ?
1.14.14.34 pentanesulfonate + FMNH2 + O2 13% of the activity with methanesulfonate Pseudomonas aeruginosa pentanal + FMN + sulfite + H2O
-
 ?
1.14.14.34 pentanesulfonate + FMNH2 + O2 13% of the activity with methanesulfonate Pseudomonas aeruginosa DSM 22644 pentanal + FMN + sulfite + H2O
-
 ?
1.14.14.34 piperazine-N,N'-bis(2-ethanesulfonic acid) + 2 FMNH2 + 2 O2 31% of the activity with methanesulfonate Pseudomonas aeruginosa 2,2'-piperazine-1,4-diyldiacetaldehyde + 2 FMN + 2 sulfite + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.34 ? x * 41600, calculated Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.14.14.34 msuD
-
 Pseudomonas aeruginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.34 Pseudomonas aeruginosa calculated
-
 6.6

Expression

EC Number Organism Comment Expression
1.14.14.34 Pseudomonas aeruginosa expression of the msu operon is repressed in the presence of sulfate, sulfite, sulfide, or cysteine down
1.14.14.34 Pseudomonas aeruginosa growth with methanesulfonate requires an intact cysB gene, and the msu operon is part of the cys regulon additional information
1.14.14.34 Pseudomonas aeruginosa expression of the msu operon is derepressed during growth with methionine or alkanesulfonates up

General Information

EC Number General Information Comment Organism
1.14.14.34 physiological function the MsuD protein catalyzes the desulfonation of alkanesulfonates, requiring oxygen and FMNH2 for the reaction, and shows highest activity with methanesulfonate. NADH-dependent flavin mononucleotide reductase MsuE provides reduced FMN for the MsuD enzyme. Putative monooxygenase MsuC is involved but not essential in the desulfonation process Pseudomonas aeruginosa