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Literature summary extracted from

  • Rong, W.; Luo, M.; Shan, T.; Wei, X.; Du, L.; Xu, H.; Zhang, Z.
    A wheat cinnamyl alcohol dehydrogenase TaCAD12 contributes to host resistance to the sharp eyespot disease (2016), Front. Plant Sci., 7, 1723.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 expressed in Escherichia coli BL21 cells Triticum aestivum
1.1.1.195 gene TaCAD12, cloned from cv. CI12633, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of GST-tagged enzyme, overexpression of the enyzem and of GFP-tagged enzyme in Triticum aestivum cv. CI12633 Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.195 0.0289
-
 coniferyl aldehyde recombinant GST-tagged enzyme, pH and temperature not specified in the publication Triticum aestivum
1.1.1.195 0.0342
-
 sinapyl aldehyde recombinant GST-tagged enzyme, pH and temperature not specified in the publication Triticum aestivum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.195 cell wall
-
 Triticum aestivum 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.195 Zn2+ require, the TaCAD12 protein contains one alcohol dehydrogenase domain (42-157 aa) with two Zn binding sites (41-63 and 76-90 aa) and one Zn-binding dehydrogenase domain (199-323 aa) Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.195 39430
-
-
 Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.195 coniferyl alcohol + NADP+ Triticum aestivum
-
 coniferyl aldehyde + NADPH + H+
-
 r
1.1.1.195 sinapyl alcohol + NADP+ Triticum aestivum
-
 sinapyl aldehyde + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Triticum aestivum
-
-
-
1.1.1.195 Triticum aestivum A0A1D6BJ11 sharp eyespot-resistant and -susceptive lines/cultivars
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.195 leaf
-
 Triticum aestivum
-
1.1.1.195 additional information transcriptional levels of TaCAD12 in sharp eyespot-resistant wheat lines are significantly higher compared with those in susceptible wheat lines Triticum aestivum
-
1.1.1.195 stem transcriptional patterns of TaCAD12 in wheat stems after Rhizoctonia cerealis inoculation analyzed by quantitative RT-PCR, overview Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.195 additional information
-
 relative enzyme activity in different sharp eyespot-resistant and -susceptive lines/cultivars Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 coniferaldehyde + NADPH + H+
-
 Triticum aestivum coniferyl alcohol + NADP+
-
 ?
1.1.1.195 coniferyl alcohol + NADP+
-
 Triticum aestivum coniferyl aldehyde + NADPH + H+
-
 r
1.1.1.195 coniferyl aldehyde + NADPH + H+
-
 Triticum aestivum coniferyl alcohol + NADP+
-
 r
1.1.1.195 sinapyl alcohol + NADP+
-
 Triticum aestivum sinapyl aldehyde + NADPH + H+
-
 r
1.1.1.195 sinapyl aldehyde + NADPH + H+
-
 Triticum aestivum sinapyl alcohol + NADP+
-
 r
1.1.1.195 sinapylaldehyde + NADPH + H+
-
 Triticum aestivum sinapyl alcohol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.195 ? x * 39430, sequence calculation Triticum aestivum
1.1.1.195 More the TaCAD12 protein contains one alcohol dehydrogenase domain (42-157 aa) with two Zn binding sites (41-63 and 76-90 aa) and one Zn-binding dehydrogenase domain (199-323 aa) with one highly conserved motif (196GLGGLG201) in CAD. The motif GLGGLG participates in binding the diophosphate group of NADP+ Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 CAD
-
 Triticum aestivum
1.1.1.195 CAD12
-
 Triticum aestivum
1.1.1.195 cinnamyl alcohol dehydrogenase
-
 Triticum aestivum
1.1.1.195 TaCAD12
-
 Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 additional information the motif GLGGLG participates in binding the diophosphate group of NADP+ Triticum aestivum
1.1.1.195 NADP+
-
 Triticum aestivum
1.1.1.195 NADPH
-
 Triticum aestivum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.195 Triticum aestivum sequence calculation
-
 7.39

Expression

EC Number Organism Comment Expression
1.1.1.195 Triticum aestivum the transcriptional levels of the enzyme in sharp eyespot-resistant wheat lines are significantly higher compared with those in susceptible wheat lines up
1.1.1.195 Triticum aestivum the enzyme is induced by infection with Rhizoctonia cerealis, a necrotrophic fungus that causes the sharp eyespot disease in wheat. Transcriptional levels of TaCAD12 in sharp eyespot-resistant wheat lines are significantly higher compared with those in susceptible wheat lines up

General Information

EC Number General Information Comment Organism
1.1.1.195 evolution TaCAD12 belongs to IV group in CAD family, phylogenetic analysis Triticum aestivum
1.1.1.195 malfunction knock-down of TaCAD12 transcript significantly represses resistance of the gene-silenced wheat plants to sharp eyespot caused by Rhizoctonia cerealis, whereas TaCAD12 overexpression markedly enhances resistance of the transgenic wheat lines to sharp eyespot. Certain defense genes (Defensin, PR10, PR17c, and Chitinase1) and monolignol biosynthesis-related genes (TaCAD1, TaCCR, and TaCOMT1) are upregulated in the TaCAD12-overexpressing wheat plants but downregulated in TaCAD12-silencing plants Triticum aestivum
1.1.1.195 physiological function enzyme overexpression markedly enhances resistance of the transgenic wheat lines to sharp eyespot caused by the necrotrophic fungus Rhizoctonia cerealis Triticum aestivum
1.1.1.195 physiological function enzyme CAD plays a role in defense responses to necrotrophic or soil-borne pathogens in wheat Triticum aestivum