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Literature summary extracted from
- Screening and identification of the levoglucosan kinase gene (lgk) from Aspergillus niger by LC-ESI-MS/MS and RT-PCR (2005), FEMS Microbiol. Lett., 251, 313-319.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.1.232 | gene lgk, DNA and amino acid seuence determination and analysis, sequence comparisons, recombinant expression in Saccharomyces cerevisiae strain H158 from pasmid pAJ401 | Aspergillus niger |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.232 | Mg2+ | required | Aspergillus niger |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.232 | 75000 | - |
gel filtration | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.232 | ATP + levoglucosan + H2O | Aspergillus niger | - |
ADP + D-glucopyranose 6-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.1.232 | Aspergillus niger | - |
gene lgk | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.1.232 | native enzyme partially by ammonium sulfate fractionation and anion exchange chromatography | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.232 | ATP + levoglucosan + H2O | - |
Aspergillus niger | ADP + D-glucopyranose 6-phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.7.1.232 | LGK | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.232 | 30 | - |
- |
Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.232 | 9.3 | - |
- |
Aspergillus niger |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.232 | ATP | - |
Aspergillus niger | |
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Release 2023.1 (January 2023)
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