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Literature summary extracted from

  • Zhang, X.; Migita, C.; Sato, M.; Sasahara, M.; Yoshida, T.
    Protein expressed by the ho2 gene of the cyanobacterium Synechocystis sp. PCC 6803 is a true heme oxygenase: Properties of the heme and enzyme complex (2005), FEBS J., 272, 1012-1022.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.20 expressed in Escherichia coli BL21(DE3) cells Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.20 28500
-
 x * 28500, calculated from amino acid sequence Synechocystis sp.
1.14.15.20 29000
-
 x * 29000, SDS-PAGE Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.20 protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Synechocystis sp.
-
 biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.20 Synechocystis sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.20 ammonium sulfate precipitation, Sephadex G-75 gel filtration , DE-52 column chromatography, and hydroxyapatite column chromatography Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.20 protoheme + reduced ferredoxin [iron-sulfur] cluster + H2O2 + H+
-
 Synechocystis sp. verdoheme + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.20 protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Synechocystis sp. biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.20 ? x * 29000, SDS-PAGE Synechocystis sp.
1.14.15.20 ? x * 28500, calculated from amino acid sequence Synechocystis sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.15.20 heme oxygenase
-
 Synechocystis sp.
1.14.15.20 HO-2 isoform Synechocystis sp.