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Literature summary extracted from

  • Umair, S.; Knight, J.S.; Bland, R.J.; Simpson, H.V.
    Molecular and biochemical characterisation of arginine kinases in Haemonchus contortus and Teladorsagia circumcincta (2013), Exp. Parasitol., 134, 362-367.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.3.3 medicine the enzyme HcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm Haemonchus contortus
2.7.3.3 medicine the enzyme TcAK is a potential vaccine candidate because of the strong antigenicity of invertebrate phosphagens and kinases and presence in metabolically active parts of the worm Teladorsagia circumcincta

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3 gene HcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Haemonchus contortus
2.7.3.3 gene TcAK, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Teladorsagia circumcincta

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.3 agmatine 65% inhibition at 2 mM Haemonchus contortus
2.7.3.3 agmatine 61% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 canavanine 81% inhibition at 2 mM Haemonchus contortus
2.7.3.3 canavanine 79% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 citrulline 17% inhibition at 2 mM Haemonchus contortus
2.7.3.3 citrulline 21% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 Creatine 11% inhibition at 2 mM Haemonchus contortus
2.7.3.3 Creatine 9% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 glutamate 15% inhibition at 2 mM Haemonchus contortus
2.7.3.3 glutamate 21% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 glycine 10% inhibition at 2 mM Haemonchus contortus
2.7.3.3 glycine 15% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 guanidine 10% inhibition at 2 mM Haemonchus contortus
2.7.3.3 guanidine 12% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 isoleucine 10% inhibition at 2 mM Haemonchus contortus
2.7.3.3 isoleucine 9% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 lysine 31% inhibition at 2 mM Haemonchus contortus
2.7.3.3 lysine 3% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 nitroarginine 30% inhibition at 2 mM Haemonchus contortus
2.7.3.3 nitroarginine 30% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 ornithine 27% inhibition at 2 mM Haemonchus contortus
2.7.3.3 ornithine 31% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 putrescine 15% inhibition at 2 mM Haemonchus contortus
2.7.3.3 putrescine 10% enzyme inhibition at 2 mM Teladorsagia circumcincta
2.7.3.3 Urea 10% inhibition at 2 mM Haemonchus contortus
2.7.3.3 Urea 13% enzyme inhibition at 2 mM Teladorsagia circumcincta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 0.3
-
 L-arginine pH 7.5, 30°C, recombinant His-tagged enzyme Teladorsagia circumcincta
2.7.3.3 0.35
-
 L-arginine pH 7.5, 30°C, recombinant His-tagged enzyme Haemonchus contortus
2.7.3.3 0.8
-
 ATP pH 7.5, 30°C, recombinant His-tagged enzyme Teladorsagia circumcincta
2.7.3.3 0.9
-
 ATP pH 7.5, 30°C, recombinant His-tagged enzyme Haemonchus contortus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.3 Cu2+ 2 mM, results in 11% activity compared to Mg2+ Teladorsagia circumcincta
2.7.3.3 Cu2+ 2 mM, results in 3% activity compared to Mg2+ Haemonchus contortus
2.7.3.3 Mg2+ 2 mM, absolutely required, no activity without Mg2+ Teladorsagia circumcincta
2.7.3.3 Mg2+ 2 mM, absolutely required, only 2% of maximal activity without Mg2+ Haemonchus contortus
2.7.3.3 Mn2+ 2 mM, results in 39% activity compared to Mg2+ Teladorsagia circumcincta
2.7.3.3 Mn2+ 2 mM, results in 41% activity compared to Mg2+ Haemonchus contortus
2.7.3.3 additional information Zn2+ cannot substitute for Mg2+ Teladorsagia circumcincta
2.7.3.3 additional information Zn2+ cannot substitute for Mg2+ Haemonchus contortus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 46000
-
-
 Teladorsagia circumcincta
2.7.3.3 46000
-
-
 Haemonchus contortus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.3 ATP + L-arginine Teladorsagia circumcincta
-
 ADP + Nomega-phospho-L-arginine
-
 r
2.7.3.3 ATP + L-arginine Haemonchus contortus
-
 ADP + Nomega-phospho-L-arginine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Haemonchus contortus K0DG62 gene HcAK
-
2.7.3.3 Teladorsagia circumcincta K0DDQ9 gene TcAK
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + L-arginine
-
 Teladorsagia circumcincta ADP + Nomega-phospho-L-arginine
-
 r
2.7.3.3 ATP + L-arginine
-
 Haemonchus contortus ADP + Nomega-phospho-L-arginine
-
 r
2.7.3.3 GTP + L-arginine GTP shows 11% of the activity with ATP Haemonchus contortus GDP + Nomega-phospho-L-arginine
-
 r
2.7.3.3 GTP + L-arginine GTP shows 7% of the activity with ATP Teladorsagia circumcincta GDP + Nomega-phospho-L-arginine
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.3.3 ? x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE Teladorsagia circumcincta
2.7.3.3 ? x * 46000, recombinant terminally His-tagged enzyme, SDS-PAGE Haemonchus contortus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.3 30
-
 assay at Teladorsagia circumcincta
2.7.3.3 30
-
 assay at Haemonchus contortus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.3 7.5
-
-
 Teladorsagia circumcincta
2.7.3.3 7.5
-
-
 Haemonchus contortus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.3 ADP
-
 Teladorsagia circumcincta
2.7.3.3 ADP
-
 Haemonchus contortus
2.7.3.3 ATP
-
 Teladorsagia circumcincta
2.7.3.3 ATP
-
 Haemonchus contortus
2.7.3.3 GTP low activity Teladorsagia circumcincta
2.7.3.3 GTP low activity Haemonchus contortus

General Information

EC Number General Information Comment Organism
2.7.3.3 evolution phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi Teladorsagia circumcincta
2.7.3.3 evolution phosphoarginine and arginine kinase are the most commonly found phosphagen and phosphagen kinase in invertebrates, such as arthropods, marine invertebrates, Haemonchus contortus larvae, the entomopathogenic nematode Steinernema carpocapsae, and the protozoan parasite Trypanosoma cruzi Haemonchus contortus