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Literature summary extracted from

  • Alhasawi, A.; Thomas, S.C.; Appanna, V.D.
    Metabolic networks to generate pyruvate, PEP and ATP from glycerol in Pseudomonas fluorescens (2016), Enzyme Microb. Technol., 85, 51-56.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.1 Mg2+ required Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate Pseudomonas fluorescens
-
 AMP + phosphoenolpyruvate + diphosphate
-
 r
2.7.9.1 ATP + pyruvate + phosphate Pseudomonas fluorescens ATCC 13525
-
 AMP + phosphoenolpyruvate + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Pseudomonas fluorescens
-
-
-
2.7.9.1 Pseudomonas fluorescens ATCC 13525
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate
-
 Pseudomonas fluorescens AMP + phosphoenolpyruvate + diphosphate
-
 r
2.7.9.1 ATP + pyruvate + phosphate
-
 Pseudomonas fluorescens ATCC 13525 AMP + phosphoenolpyruvate + diphosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.9.1 PPDK
-
 Pseudomonas fluorescens
2.7.9.1 pyruvate phosphate dikinase
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.1 AMP
-
 Pseudomonas fluorescens
2.7.9.1 ATP
-
 Pseudomonas fluorescens

Expression

EC Number Organism Comment Expression
2.7.9.1 Pseudomonas fluorescens H2O2 induces the enzyme expression up

General Information

EC Number General Information Comment Organism
2.7.9.1 metabolism metabolic networks involved in its transformation into pyruvate, phosphoenolpyruvate (PEP) and ATP, overview. exposed to hydrogen peroxide in a mineral medium with glycerol as the sole carbon source, the microbe reconfigures its metabolism to generate ATP primarily via substrate-level phosphorylation. This alternative ATP-producing stratagem results in the synthesis of copious amounts of PEP and pyruvate. The production of these metabolites is mediated via the enhanced activities of such enzymes as pyruvatecarboxylase and phosphoenolpyruvate carboxylase. The high energy PEP is subsequentlyconverted into ATP with the aid of pyruvate phosphate dikinase, phosphoenolpyruvate synthase, and pyruvate kinase with the concomitant formation of pyruvate. The participation of the phosphotransfer enzymes like adenylate kinase and acetate kinase ensures the efficiency of this O2-independent energy-generating machinery. The increased activity of glycerol dehydrogenase in the stressed bacteria provides the necessary precursors to fuel this process. This H2O2-induced anaerobic life-style fortuitously evokes metabolic networks to an effective pathway that can be harnessed into the synthesis of ATP, PEP, and pyruvate Pseudomonas fluorescens