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Literature summary extracted from

  • Tadrowski, S.; Pedroso, M.M.; Sieber, V.; Larrabee, J.A.; Guddat, L.W.; Schenk, G.
    Metal ions play an essential catalytic role in the mechanism of ketol-acid reductoisomerase (2016), Chemistry, 22, 7427-7436.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 Mg2+ dependent on Escherichia coli
1.1.1.86 Mg2+ dependent on Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ Escherichia coli
-
 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
 r
1.1.1.86 (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ Oryza sativa
-
 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Escherichia coli
-
-
-
1.1.1.86 Oryza sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
 Escherichia coli (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
 r
1.1.1.86 (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
 Oryza sativa (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
 r
1.1.1.86 2-acetolactate + NADPH + H+
-
 Escherichia coli 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 ?
1.1.1.86 2-acetolactate + NADPH + H+
-
 Oryza sativa 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 ?
1.1.1.86 3-hydroxypyruvate + NADPH + H+
-
 Escherichia coli ?
-
 ?
1.1.1.86 3-hydroxypyruvate + NADPH + H+
-
 Oryza sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.86 KARI
-
 Escherichia coli
1.1.1.86 KARI
-
 Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.86 1.2
-
 2-acetolactate at pH 8.0, temperature not specified in the publication Escherichia coli
1.1.1.86 1.4
-
 2-acetolactate at pH 8.0, temperature not specified in the publication Oryza sativa
1.1.1.86 4.5
-
 3-hydroxypyruvate at pH 8.0, temperature not specified in the publication Oryza sativa
1.1.1.86 26
-
 3-hydroxypyruvate at pH 8.0, temperature not specified in the publication Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.86 7 9
-
 Escherichia coli
1.1.1.86 8
-
-
 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADP+
-
 Escherichia coli
1.1.1.86 NADP+
-
 Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.86 2.4
-
 2-acetolactate at pH 8.0, temperature not specified in the publication Escherichia coli
1.1.1.86 3.3
-
 3-hydroxypyruvate at pH 8.0, temperature not specified in the publication Escherichia coli
1.1.1.86 4.16
-
 3-hydroxypyruvate at pH 8.0, temperature not specified in the publication Oryza sativa
1.1.1.86 120
-
 2-acetolactate at pH 8.0, temperature not specified in the publication Oryza sativa