EC Number | Application | Comment | Organism |
---|---|---|---|
2.5.1.63 | biotechnology | the enzyme is used for [18F]-radiolabelling of bioactive peptides, overview | Streptomyces cattleya |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.63 | gene flA, genetic structure and organization | Nocardia brasiliensis |
2.5.1.63 | gene flA, genetic structure and organization, the flA gene is located in close proximity to the corresponding flB gene | Streptomyces sp. |
2.5.1.63 | gene flA, genetic structure and organization, the flA gene is located in close proximity to the corresponding flB gene | Actinoplanes sp. |
2.5.1.63 | gene flA, genetic structure and organization, the flA gene is located in close proximity to the corresponding flB gene | Streptomyces xinghaiensis |
2.5.1.63 | gene flA, located at the center of the 10 kb gene Spencer cluster, genetic structure and organization, the f lA gene is located in close proximity to the corresponding f lB gene. Funtional recombinant expression in Salinispora tropica using gene replacement and replacing the gene salL that encodes chlorinase, EC 2.5.1.94, by gene flA from Streptomaces cattleya. The mutated Salinispora tropica strain produces fluorosalinosporamide | Streptomyces cattleya |
2.5.1.94 | gene salL, the gene is located in the middle of this salinosporamide A cluster | Salinispora tropica |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.63 | structure modelling of the fluoride ion/SAM prereaction complex at the active site of the fluorinase immediately prior to SN2 substitution, fluoride ion is modeled into the SAM-fluorinase cocomplex, which is solved by X-ray crystallography, overview | Streptomyces cattleya |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.63 | additional information | the enzyme is used for [18F]-radiolabelling of bioactive peptides, overview | Streptomyces cattleya |
2.5.1.94 | additional information | gene replacement of gene salL from Salinispora tropica by gene flA, EC 2.5.1.63, encoding fluorinase from Streptomaces cattleya, the recombinant fluorinase is functional in Salinispora tropica | Salinispora tropica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.63 | 0.01 | - |
S-adenosyl-L-methionine | about, during turnover, pH and temperature not specified in the publication | Streptomyces cattleya | |
2.5.1.63 | 10 | - |
fluoride | about, during turnover, pH and temperature not specified in the publication | Streptomyces cattleya |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.63 | S-adenosyl-L-methionine + fluoride | Nocardia brasiliensis | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | Streptomyces sp. | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | Actinoplanes sp. | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | Streptomyces xinghaiensis | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | Streptomyces cattleya | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
r | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | Streptomyces xinghaiensis NRRL B-24674 | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | Actinoplanes sp. N902-109 | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.94 | S-adenosyl-L-methionine + chloride | Salinispora tropica | - |
5'-deoxy-5'-chloroadenosine + L-methionine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.63 | Actinoplanes sp. | R4LHX8 | gene flA | - |
2.5.1.63 | Actinoplanes sp. N902-109 | R4LHX8 | gene flA | - |
2.5.1.63 | Nocardia brasiliensis | - |
a South American hospital pathogen, gene flA | - |
2.5.1.63 | Streptomyces cattleya | Q70GK9 | gene flA | - |
2.5.1.63 | Streptomyces sp. | W0W999 | solated in 2011 from Ghana, gene flA | - |
2.5.1.63 | Streptomyces xinghaiensis | A0A068VNW5 | gene flA4 | - |
2.5.1.63 | Streptomyces xinghaiensis NRRL B-24674 | A0A068VNW5 | gene flA4 | - |
2.5.1.94 | Salinispora tropica | - |
a marine organism, gene salL | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.63 | S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine | SN2 substitution reaction mechanism, where fluoride ion mediates a nucleophilic attack to the 5'-carbon of the ribose ring of S-adenosyl-L-methionine to give 5'-deoxy-5'-fluoroadenosine. The reaction proceeds with an inversion of configuration at the C-5'carbon | Streptomyces cattleya |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.63 | S-adenosyl-L-methionine + fluoride | - |
Nocardia brasiliensis | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | - |
Streptomyces sp. | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | - |
Actinoplanes sp. | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | - |
Streptomyces xinghaiensis | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
r | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | - |
Streptomyces xinghaiensis NRRL B-24674 | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | - |
Actinoplanes sp. N902-109 | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
2.5.1.94 | additional information | chlorinase does not accept fluoride ion as a substrate | Salinispora tropica | ? | - |
? | |
2.5.1.94 | S-adenosyl-L-methionine + chloride | - |
Salinispora tropica | 5'-deoxy-5'-chloroadenosine + L-methionine | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.63 | fluorinase | - |
Nocardia brasiliensis |
2.5.1.63 | fluorinase | - |
Streptomyces sp. |
2.5.1.63 | fluorinase | - |
Actinoplanes sp. |
2.5.1.63 | fluorinase | - |
Streptomyces xinghaiensis |
2.5.1.63 | fluorinase | - |
Streptomyces cattleya |
2.5.1.94 | chlorinase | - |
Salinispora tropica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.63 | 25 | - |
assay at | Streptomyces cattleya |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.5.1.63 | Streptomyces cattleya | the fluorometabolite pathway is upregulated by increased fluoride ion levels, perhaps due to upregulation of f lG encoding a putative DNA binding regulatory protein | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.63 | metabolism | the enzyme is involved in secondary metabolism, delivering bioactive fluorinated natural products when the bacterium is grown in a medium containing fluoride | Streptomyces cattleya |
2.5.1.94 | metabolism | the enzyme is responsible for salinosporamide-A biosynthesis | Salinispora tropica |