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Literature summary extracted from

  • Achbergerova, L.; Nahalka, J.
    Degradation of polyphosphates by polyphosphate kinases from Ruegeria pomeroyi (2014), Biotechnol. Lett., 36, 2029-2035.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 gene SPO0224, DNA and amino acid sequence determination and analysis, recombinant expression of Strep-tagged isozyme PPK2-3 in Escherichia coli strain BL21(DE3)-T1 Ruegeria pomeroyi
2.7.4.1 gene SPO1256, DNA and amino acid sequence determination and analysis, recombinant expression of STrep-tagged isoyzme PPK2-2 in Escherichia coli strain BL21(DE3)-T1 Ruegeria pomeroyi
2.7.4.1 gene SPO1727, DNA and amino acid sequence determination and analysis, recombinant expression of Strep-tagged isozyme PPK2-1 in Escherichia coli strain BL21(DE3)-T1 Ruegeria pomeroyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 Co2+ complete inhibition; complete inhibition; complete inhibition Ruegeria pomeroyi
2.7.4.1 EDTA low inhibition; low inhibition Ruegeria pomeroyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 1.2
-
 GDP pH 8.0, 30°C, recombinant isozyme PPK2-1 Ruegeria pomeroyi
2.7.4.1 2.5
-
 ADP pH 8.0, 30°C, recombinant isozyme PPK2-2 Ruegeria pomeroyi
2.7.4.1 3.6
-
 UDP pH 8.0, 30°C, recombinant isozyme PPK2-3 Ruegeria pomeroyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Ca2+ activates Ruegeria pomeroyi
2.7.4.1 Ca2+ activates, best cation Ruegeria pomeroyi
2.7.4.1 Mg2+ activates Ruegeria pomeroyi
2.7.4.1 Mg2+ activates, best cation Ruegeria pomeroyi
2.7.4.1 Mn2+ activates Ruegeria pomeroyi
2.7.4.1 Mn2+ activates, best cation Ruegeria pomeroyi
2.7.4.1 additional information enzyme SPO1727 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview Ruegeria pomeroyi
2.7.4.1 additional information isozyme SPO0224 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview Ruegeria pomeroyi
2.7.4.1 additional information isozyme SPO125 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview Ruegeria pomeroyi
2.7.4.1 Ni2+ activates Ruegeria pomeroyi
2.7.4.1 Zn2+ activates Ruegeria pomeroyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.1 34750
-
 calculated from sequence, SDS-PAGE Ruegeria pomeroyi
2.7.4.1 36720
-
 calculated from sequence, SDS-PAGE Ruegeria pomeroyi
2.7.4.1 39950
-
 calculated from sequence, SDS-PAGE Ruegeria pomeroyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ADP + (phosphate)n Ruegeria pomeroyi
-
 ATP + (phosphate)n-1
-
 ?
2.7.4.1 ADP + (phosphate)n Ruegeria pomeroyi ATCC 700808
-
 ATP + (phosphate)n-1
-
 ?
2.7.4.1 CDP + (phosphate)n Ruegeria pomeroyi
-
 CTP + (phosphate)n-1
-
 ?
2.7.4.1 CDP + (phosphate)n Ruegeria pomeroyi ATCC 700808
-
 CTP + (phosphate)n-1
-
 ?
2.7.4.1 GDP + (phosphate)n Ruegeria pomeroyi
-
 GTP + (phosphate)n-1
-
 ?
2.7.4.1 GDP + (phosphate)n Ruegeria pomeroyi ATCC 700808
-
 GTP + (phosphate)n-1
-
 ?
2.7.4.1 UDP + (phosphate)n Ruegeria pomeroyi
-
 UTP + (phosphate)n-1
-
 ?
2.7.4.1 UDP + (phosphate)n Ruegeria pomeroyi ATCC 700808
-
 UTP + (phosphate)n-1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Ruegeria pomeroyi Q5LSN8 gene SPO1727, isozyme PPK2-1
-
2.7.4.1 Ruegeria pomeroyi Q5LU04 gene SPO1256, isozyme PPK2-2
-
2.7.4.1 Ruegeria pomeroyi Q5LX16 gene SPO0224, isozyme PPK2-3
-
2.7.4.1 Ruegeria pomeroyi ATCC 700808 Q5LSN8 gene SPO1727, isozyme PPK2-1
-
2.7.4.1 Ruegeria pomeroyi ATCC 700808 Q5LU04 gene SPO1256, isozyme PPK2-2
-
2.7.4.1 Ruegeria pomeroyi ATCC 700808 Q5LX16 gene SPO0224, isozyme PPK2-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 recombinant Strep-tagged isozyme PPK2-1 from Escherichia coli strain BL21(DE3) by streptavidin affinity chromatography Ruegeria pomeroyi
2.7.4.1 recombinant Strep-tagged isozyme PPK2-2 from Escherichia coli strain BL21(DE3) by streptavidin affinity chromatography Ruegeria pomeroyi
2.7.4.1 recombinant Strep-tagged isozyme PPK2-3 from Escherichia coli strain BL21(DE3) by streptavidin affinity chromatography Ruegeria pomeroyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + (phosphate)n
-
 Ruegeria pomeroyi ATP + (phosphate)n-1
-
 ?
2.7.4.1 ADP + (phosphate)n
-
 Ruegeria pomeroyi ATCC 700808 ATP + (phosphate)n-1
-
 ?
2.7.4.1 CDP + (phosphate)n
-
 Ruegeria pomeroyi CTP + (phosphate)n-1
-
 ?
2.7.4.1 CDP + (phosphate)n
-
 Ruegeria pomeroyi ATCC 700808 CTP + (phosphate)n-1
-
 ?
2.7.4.1 GDP + (phosphate)n
-
 Ruegeria pomeroyi GTP + (phosphate)n-1
-
 ?
2.7.4.1 GDP + (phosphate)n
-
 Ruegeria pomeroyi ATCC 700808 GTP + (phosphate)n-1
-
 ?
2.7.4.1 additional information all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity Ruegeria pomeroyi ?
-
 ?
2.7.4.1 additional information all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity Ruegeria pomeroyi ATCC 700808 ?
-
 ?
2.7.4.1 UDP + (phosphate)n
-
 Ruegeria pomeroyi UTP + (phosphate)n-1
-
 ?
2.7.4.1 UDP + (phosphate)n
-
 Ruegeria pomeroyi ATCC 700808 UTP + (phosphate)n-1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.1 monomer 1 * 34750, recombinant PPK2-3, sequence calculation and SDS-PAGE Ruegeria pomeroyi
2.7.4.1 monomer 1 * 36720, recombinant PPK2-1, sequence calculation and SDS-PAGE Ruegeria pomeroyi
2.7.4.1 monomer 1 * 39950, recombinant PPK2-2, sequence calculation and SDS-PAGE Ruegeria pomeroyi

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 polyphosphate kinase 2
-
 Ruegeria pomeroyi
2.7.4.1 PPK2
-
 Ruegeria pomeroyi
2.7.4.1 PPK2-1
-
 Ruegeria pomeroyi
2.7.4.1 PPK2-2
-
 Ruegeria pomeroyi
2.7.4.1 PPK2-3
-
 Ruegeria pomeroyi
2.7.4.1 SPO0224
-
 Ruegeria pomeroyi
2.7.4.1 SPO1256
-
 Ruegeria pomeroyi
2.7.4.1 SPO1727
-
 Ruegeria pomeroyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 30
-
 assay at Ruegeria pomeroyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.1 4.18
-
 GDP pH 8.0, 30°C, recombinant isozyme PPK2-1 Ruegeria pomeroyi
2.7.4.1 4.9
-
 UDP pH 8.0, 30°C, recombinant isozyme PPK2-3 Ruegeria pomeroyi
2.7.4.1 5.22
-
 ADP pH 8.0, 30°C, recombinant isozyme PPK2-2 Ruegeria pomeroyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 8
-
-
 Ruegeria pomeroyi
2.7.4.1 8
-
 optimum 1 Ruegeria pomeroyi
2.7.4.1 9
-
-
 Ruegeria pomeroyi
2.7.4.1 10
-
 optimum 2 Ruegeria pomeroyi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.1 5 11 activity range, profile overview Ruegeria pomeroyi

General Information

EC Number General Information Comment Organism
2.7.4.1 evolution in general, all PPKs are ancient enzymes, and phylogenetic analysis indicates that the polyphosphate degradation process is older than polyphosphate synthesis. Additionally, PPK2 proteins are older than PPK1 enzymes Ruegeria pomeroyi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.1 1.37
-
 UDP pH 8.0, 30°C, recombinant isozyme PPK2-3 Ruegeria pomeroyi
2.7.4.1 2.08
-
 ADP pH 8.0, 30°C, recombinant isozyme PPK2-2 Ruegeria pomeroyi
2.7.4.1 3.48
-
 GDP pH 8.0, 30°C, recombinant isozyme PPK2-1 Ruegeria pomeroyi