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Literature summary extracted from

  • Wu, H.; Bennett, G.N.; San, K.Y.
    Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation (2015), Biotechnol. Bioeng., 112, 1720-1726.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.93 recombinant expression in Escherichia coli coenzyme Q8 biosynthesis-deficient strains, HW108 and HW109, which contain mutations in ubiE and ubiG, respectively. Gene lePGT-1 gene is amplified and cloned into the expression vector, pBAD33, to form pBlePGT, the fine-tuning of the expression of lePGT-1 in the ubiquinone-deficient strains, HW108 and HW109, to control the electron transfer chain is investigated by adding different concentrations of the inducer arabinose at 0-20 mM under fully aerobic conditions Lithospermum erythrorhizon

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.93 membrane
-
 Lithospermum erythrorhizon 16020
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.93 geranyl diphosphate + 4-hydroxybenzoate Lithospermum erythrorhizon
-
 3-geranyl-4-hydroxybenzoate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.93 Lithospermum erythrorhizon Q8W405 gene PGT-1 or lepgt
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.93 geranyl diphosphate + 4-hydroxybenzoate
-
 Lithospermum erythrorhizon 3-geranyl-4-hydroxybenzoate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.93 diphosphate:4-hydroxybenzoate geranyltransferase
-
 Lithospermum erythrorhizon
2.5.1.93 geranyl diphosphate:4-hydroxybenzoate 3-geranyltransferase
-
 Lithospermum erythrorhizon
2.5.1.93 lepgt
-
 Lithospermum erythrorhizon
2.5.1.93 PGT-1
-
 Lithospermum erythrorhizon

General Information

EC Number General Information Comment Organism
2.5.1.93 metabolism the reaction catalyzed by lePGT-1 plays as a competing pathway on two substrates of the Q8 synthesis pathway, namely at IPP and 4-hydroxybenzoate Lithospermum erythrorhizon
2.5.1.93 physiological function the enzyme is part of a metabolic strategy to further controll the respiratory levels in CoQ8 biosynthesis-deficient Escherichia coli strains, this metabolic strategy can lead to fine-tuning of aerobic lactate accumulation. The lactate and acetate concentrations increase with increasing enzyme levels, as does the cell density, while glucose concentration decreases, overview Lithospermum erythrorhizon