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Literature summary extracted from

  • Ravilious, G.; Herrmann, J.; Lee, S.; Westfall, C.; Jez, J.
    Kinetic mechanism of the dimeric ATP sulfurylase from plants (2013), Biosci. Rep., 33, 585-591.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.4 recombinant expression of His-tagged enzyme in Escherichia coli BL21(DE3) Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.4 Mg2+ required Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.4 ATP + sulfate Glycine max
-
 diphosphate + adenylyl sulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Glycine max Q8SAG1 ATP sulfurylase isoform 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4 recombinant His-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration Glycine max

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.4 ATP + sulfate = diphosphate + adenylyl sulfate analysis of the bi bi substrate kinetic mechanism of GmATPS1. The enzyme shows a kinetic mechanism in which ATP and APS are the first substrates bound in the forward and reverse reactions, respectively Glycine max
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Glycine max diphosphate + adenylyl sulfate
-
 r
2.7.7.4 additional information no binding of diphosphate to enzyme GmATPS1 Glycine max ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.4 ATP sulfurylase
-
 Glycine max
2.7.7.4 ATP sulfurylase isoform 1
-
 Glycine max
2.7.7.4 ATP: sulfate adenylyl transferase
-
 Glycine max
2.7.7.4 ATPS1
-
 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.4 ATP
-
 Glycine max

General Information

EC Number General Information Comment Organism
2.7.7.4 evolution in plants, gene families encode multiple isoforms of ATP sulfurylase with varied expression patterns and organelle localization Glycine max
2.7.7.4 metabolism the first enzymatic reaction in the sulfur assimilation pathway of plants is the non-reductive adenylation of sulfate catalysed by ATP sulfurylase to yield adenylyl sulfate, APS, and diphosphate. The enzyme also catalyzes the next step, producing ADP and 3'-phosphoadenylyl sulfate from ATP and adenylyl sulfate, cf. EC 2.7.1.25 Glycine max
2.7.7.4 physiological function the sulfur assimilation pathway provides sulfide for a range of biosynthetic pathways that supply methionine, glutathione, iron–sulfur clusters, vitamin cofactors such as biotin and thiamin, and multiple specialized metabolites such as glucosinolates Glycine max