BRENDA - Enzyme Database

Identification and characterization of a mycobacterial NAD+-dependent alcohol dehydrogenase with superior reduction of diacetyl to (S)-acetoin

Takeda, M.; Anamizu, S.; Motomatsu, S.; Chen, X.; Thapa Chhetri, R.; Biosci. Biotechnol. Biochem. 78, 1879-1886 (2014)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.02
-
NAD+
cosubstrate butan-1-ol, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
0.09
-
NADH
cosubstrate butanal, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
0.48
-
Butanal
cosubstrate NADH, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
5.65
-
butan-1-ol
cosubstrate NAD+, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.76
42.2
-
(R,S)-acetoin
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
0.05
-
NADH
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
4.47
-
diacetyl
pH 7.0, 30°C
Mycobacterium sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
36000
-
-
Mycobacterium sp.
1.1.1.1
150000
-
gel filtration
Mycobacterium sp.
1.1.1.76
36000
-
-
Mycobacterium sp.
1.1.1.76
150000
-
gel filtration
Mycobacterium sp.
1.1.1.304
36000
-
-
Mycobacterium sp.
1.1.1.304
150000
-
gel filtration
Mycobacterium sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.1
Mycobacterium sp.
W8VSK8
-
-
1.1.1.1
Mycobacterium sp. B-009
W8VSK8
-
-
1.1.1.76
Mycobacterium sp.
W8VSK8
-
-
1.1.1.76
Mycobacterium sp. B-009
W8VSK8
-
-
1.1.1.304
Mycobacterium sp.
W8VSK8
-
-
1.1.1.304
Mycobacterium sp. B-009
W8VSK8
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.1
1,5-pentanediol + NAD+
18% of the activity with butan-1-ol
737865
Mycobacterium sp.
? + NADH + H+
-
-
-
?
1.1.1.1
1,5-pentanediol + NAD+
18% of the activity with butan-1-ol
737865
Mycobacterium sp. B-009
? + NADH + H+
-
-
-
?
1.1.1.1
butan-1-ol + NAD+
best substrate
737865
Mycobacterium sp.
butanal + NADH + H+
-
-
-
r
1.1.1.1
butanal + NADH + H+
-
737865
Mycobacterium sp.
butan-1-ol + NAD+
-
-
-
r
1.1.1.1
ethanol + NAD+
12% of the activity with butan-1-ol
737865
Mycobacterium sp.
acetaldehyde + NADH + H+
-
-
-
r
1.1.1.1
ethanol + NAD+
12% of the activity with butan-1-ol
737865
Mycobacterium sp. B-009
acetaldehyde + NADH + H+
-
-
-
r
1.1.1.1
additional information
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
737865
Mycobacterium sp.
?
-
-
-
-
1.1.1.1
additional information
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
737865
Mycobacterium sp. B-009
?
-
-
-
-
1.1.1.1
propan-1-ol + NAD+
55% of the activity with butan-1-ol
737865
Mycobacterium sp.
propanal + NADH + H+
-
-
-
r
1.1.1.1
propan-1-ol + NAD+
55% of the activity with butan-1-ol
737865
Mycobacterium sp. B-009
propanal + NADH + H+
-
-
-
r
1.1.1.1
propan-2-ol + NAD+
65% of the activity with butan-1-ol
737865
Mycobacterium sp.
acetone + NADH + H+
-
-
-
r
1.1.1.1
propan-2-ol + NAD+
65% of the activity with butan-1-ol
737865
Mycobacterium sp. B-009
acetone + NADH + H+
-
-
-
r
1.1.1.76
(2S,3S)-2,3-butanediol + NAD+
-
737865
Mycobacterium sp.
(R,S)-acetoin + NADH + H+
selective catalysis of S,S- and meso-butanediol, but not R,R-butanediol
-
-
r
1.1.1.76
(R,S)-acetoin + NADH + H+
-
737865
Mycobacterium sp.
(2S,3S)-2,3-butanediol + meso-2,3-butanediol + NAD+
-
-
-
r
1.1.1.76
meso-2,3-butanediol + NAD+
-
737865
Mycobacterium sp.
(R,S)-acetoin + NADH + H+
selective catalysis of S,S- and meso-butanediol, but not R,R-butanediol
-
-
r
1.1.1.76
additional information
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
737865
Mycobacterium sp.
?
-
-
-
-
1.1.1.304
diacetyl + NADH + H+
-
737865
Mycobacterium sp.
(S)-acetoin + NAD+
-
-
-
?
1.1.1.304
additional information
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
737865
Mycobacterium sp.
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.1
tetramer
4 * 36000, SDS-PAGE, 4 * 35971, calculated
Mycobacterium sp.
1.1.1.76
tetramer
4 * 36000, SDS-PAGE, 4 * 35971, calculated
Mycobacterium sp.
1.1.1.304
tetramer
4 * 36000, SDS-PAGE, 4 * 35971, calculated
Mycobacterium sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
1
-
NAD+
cosubstrate butan-1-ol, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
2
-
butan-1-ol
cosubstrate NAD+, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
102
-
Butanal
cosubstrate NADH, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
153
-
NADH
cosubstrate butanal, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.76
6
-
(R,S)-acetoin
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
110
-
NADH
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
163
-
diacetyl
pH 7.0, 30°C
Mycobacterium sp.
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.1.1
Mycobacterium sp.
isoelectric focusing
-
4.8
1.1.1.1
Mycobacterium sp.
calculated
-
5.1
1.1.1.76
Mycobacterium sp.
isoelectric focusing
-
4.8
1.1.1.76
Mycobacterium sp.
calculated
-
5.1
1.1.1.304
Mycobacterium sp.
isoelectric focusing
-
4.8
1.1.1.304
Mycobacterium sp.
calculated
-
5.1
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.02
-
NAD+
cosubstrate butan-1-ol, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
0.09
-
NADH
cosubstrate butanal, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
0.48
-
Butanal
cosubstrate NADH, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
5.65
-
butan-1-ol
cosubstrate NAD+, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.76
42.2
-
(R,S)-acetoin
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
0.05
-
NADH
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
4.47
-
diacetyl
pH 7.0, 30°C
Mycobacterium sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
36000
-
-
Mycobacterium sp.
1.1.1.1
150000
-
gel filtration
Mycobacterium sp.
1.1.1.76
36000
-
-
Mycobacterium sp.
1.1.1.76
150000
-
gel filtration
Mycobacterium sp.
1.1.1.304
36000
-
-
Mycobacterium sp.
1.1.1.304
150000
-
gel filtration
Mycobacterium sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.1
1,5-pentanediol + NAD+
18% of the activity with butan-1-ol
737865
Mycobacterium sp.
? + NADH + H+
-
-
-
?
1.1.1.1
1,5-pentanediol + NAD+
18% of the activity with butan-1-ol
737865
Mycobacterium sp. B-009
? + NADH + H+
-
-
-
?
1.1.1.1
butan-1-ol + NAD+
best substrate
737865
Mycobacterium sp.
butanal + NADH + H+
-
-
-
r
1.1.1.1
butanal + NADH + H+
-
737865
Mycobacterium sp.
butan-1-ol + NAD+
-
-
-
r
1.1.1.1
ethanol + NAD+
12% of the activity with butan-1-ol
737865
Mycobacterium sp.
acetaldehyde + NADH + H+
-
-
-
r
1.1.1.1
ethanol + NAD+
12% of the activity with butan-1-ol
737865
Mycobacterium sp. B-009
acetaldehyde + NADH + H+
-
-
-
r
1.1.1.1
additional information
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
737865
Mycobacterium sp.
?
-
-
-
-
1.1.1.1
additional information
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
737865
Mycobacterium sp. B-009
?
-
-
-
-
1.1.1.1
propan-1-ol + NAD+
55% of the activity with butan-1-ol
737865
Mycobacterium sp.
propanal + NADH + H+
-
-
-
r
1.1.1.1
propan-1-ol + NAD+
55% of the activity with butan-1-ol
737865
Mycobacterium sp. B-009
propanal + NADH + H+
-
-
-
r
1.1.1.1
propan-2-ol + NAD+
65% of the activity with butan-1-ol
737865
Mycobacterium sp.
acetone + NADH + H+
-
-
-
r
1.1.1.1
propan-2-ol + NAD+
65% of the activity with butan-1-ol
737865
Mycobacterium sp. B-009
acetone + NADH + H+
-
-
-
r
1.1.1.76
(2S,3S)-2,3-butanediol + NAD+
-
737865
Mycobacterium sp.
(R,S)-acetoin + NADH + H+
selective catalysis of S,S- and meso-butanediol, but not R,R-butanediol
-
-
r
1.1.1.76
(R,S)-acetoin + NADH + H+
-
737865
Mycobacterium sp.
(2S,3S)-2,3-butanediol + meso-2,3-butanediol + NAD+
-
-
-
r
1.1.1.76
meso-2,3-butanediol + NAD+
-
737865
Mycobacterium sp.
(R,S)-acetoin + NADH + H+
selective catalysis of S,S- and meso-butanediol, but not R,R-butanediol
-
-
r
1.1.1.76
additional information
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
737865
Mycobacterium sp.
?
-
-
-
-
1.1.1.304
diacetyl + NADH + H+
-
737865
Mycobacterium sp.
(S)-acetoin + NAD+
-
-
-
?
1.1.1.304
additional information
enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities
737865
Mycobacterium sp.
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.1
tetramer
4 * 36000, SDS-PAGE, 4 * 35971, calculated
Mycobacterium sp.
1.1.1.76
tetramer
4 * 36000, SDS-PAGE, 4 * 35971, calculated
Mycobacterium sp.
1.1.1.304
tetramer
4 * 36000, SDS-PAGE, 4 * 35971, calculated
Mycobacterium sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
1
-
NAD+
cosubstrate butan-1-ol, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
2
-
butan-1-ol
cosubstrate NAD+, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
102
-
Butanal
cosubstrate NADH, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
153
-
NADH
cosubstrate butanal, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.76
6
-
(R,S)-acetoin
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
110
-
NADH
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
163
-
diacetyl
pH 7.0, 30°C
Mycobacterium sp.
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.1.1
Mycobacterium sp.
isoelectric focusing
-
4.8
1.1.1.1
Mycobacterium sp.
calculated
-
5.1
1.1.1.76
Mycobacterium sp.
isoelectric focusing
-
4.8
1.1.1.76
Mycobacterium sp.
calculated
-
5.1
1.1.1.304
Mycobacterium sp.
isoelectric focusing
-
4.8
1.1.1.304
Mycobacterium sp.
calculated
-
5.1
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.3
-
butan-1-ol
cosubstrate NAD+, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
80
-
NAD+
cosubstrate butan-1-ol, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
210
-
Butanal
cosubstrate NADH, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
2000
-
NADH
cosubstrate butanal, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.76
0.1
-
(R,S)-acetoin
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
36.4
-
diacetyl
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
210
-
NADH
pH 7.0, 30°C
Mycobacterium sp.
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.3
-
butan-1-ol
cosubstrate NAD+, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
80
-
NAD+
cosubstrate butan-1-ol, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
210
-
Butanal
cosubstrate NADH, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.1
2000
-
NADH
cosubstrate butanal, pH 7.0, 30°C
Mycobacterium sp.
1.1.1.76
0.1
-
(R,S)-acetoin
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
36.4
-
diacetyl
pH 7.0, 30°C
Mycobacterium sp.
1.1.1.304
210
-
NADH
pH 7.0, 30°C
Mycobacterium sp.