BRENDA - Enzyme Database

Plausible novel ribose metabolism catalyzed by enzymes of the methionine salvage pathway in Bacillus subtilis

Nakano, T.; Saito, Y.; Yokota, A.; Ashida, H.; Biosci. Biotechnol. Biochem. 77, 1104-1107 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.100
gene mtnK, encoded in the mtnKA operon
Bacillus subtilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.100
additional information
-
additional information
the Km for ribose is 80times higher than for S-methyl-5-thio-D-ribose
Bacillus subtilis
2.7.1.100
0.06
-
S-methyl-5-thio-D-ribose
pH 8.0, 25°C
Bacillus subtilis
2.7.1.100
4.8
-
D-ribose
pH 8.0, 25°C
Bacillus subtilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.100
Mg2+
required
Bacillus subtilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.100
ATP + S-methyl-5-thio-D-ribose
Bacillus subtilis
physiological substrate
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.100
Bacillus subtilis
-
gene mtnK
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.100
ATP + D-ribose
-
737858
Bacillus subtilis
ADP + D-ribose 1-phosphate
-
-
-
?
2.7.1.100
ATP + S-methyl-5-thio-D-ribose
best substrate
737858
Bacillus subtilis
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
-
-
?
2.7.1.100
ATP + S-methyl-5-thio-D-ribose
physiological substrate
737858
Bacillus subtilis
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
-
-
?
2.7.1.100
additional information
the enzyme has significant ribose kinase activity, it has a hydrophobic pocket that specifically recognizes the methylthio group of the substrate. MTK shows a 9fold higher Vmax for ribose kinase activity than for S-methyl-5-thio-D-ribose kinase activity, but also has a several fold higher Km for ribose
737858
Bacillus subtilis
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.100
25
35
ribose kinase activity
Bacillus subtilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.100
7
8.5
ribose kinase activity
Bacillus subtilis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.100
ATP
-
Bacillus subtilis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.100
gene mtnK, encoded in the mtnKA operon
Bacillus subtilis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.100
ATP
-
Bacillus subtilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.100
additional information
-
additional information
the Km for ribose is 80times higher than for S-methyl-5-thio-D-ribose
Bacillus subtilis
2.7.1.100
0.06
-
S-methyl-5-thio-D-ribose
pH 8.0, 25°C
Bacillus subtilis
2.7.1.100
4.8
-
D-ribose
pH 8.0, 25°C
Bacillus subtilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.100
Mg2+
required
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.100
ATP + S-methyl-5-thio-D-ribose
Bacillus subtilis
physiological substrate
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.100
ATP + D-ribose
-
737858
Bacillus subtilis
ADP + D-ribose 1-phosphate
-
-
-
?
2.7.1.100
ATP + S-methyl-5-thio-D-ribose
best substrate
737858
Bacillus subtilis
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
-
-
?
2.7.1.100
ATP + S-methyl-5-thio-D-ribose
physiological substrate
737858
Bacillus subtilis
ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
-
-
?
2.7.1.100
additional information
the enzyme has significant ribose kinase activity, it has a hydrophobic pocket that specifically recognizes the methylthio group of the substrate. MTK shows a 9fold higher Vmax for ribose kinase activity than for S-methyl-5-thio-D-ribose kinase activity, but also has a several fold higher Km for ribose
737858
Bacillus subtilis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.100
25
35
ribose kinase activity
Bacillus subtilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.100
7
8.5
ribose kinase activity
Bacillus subtilis
General Information
EC Number
General Information
Commentary
Organism
2.7.1.100
evolution
although most bacteria, yeasts, and animals have the complete methionine salvage pathway, MSP enzymes, these organisms have 5-methylthioadenosine phosphorylase, MtnP, instead of MtnK
Bacillus subtilis
2.7.1.100
metabolism
the enzyme catalyze the first reaction of a ribose metabolic pathway, overview
Bacillus subtilis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.100
evolution
although most bacteria, yeasts, and animals have the complete methionine salvage pathway, MSP enzymes, these organisms have 5-methylthioadenosine phosphorylase, MtnP, instead of MtnK
Bacillus subtilis
2.7.1.100
metabolism
the enzyme catalyze the first reaction of a ribose metabolic pathway, overview
Bacillus subtilis