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Literature summary extracted from

  • Kumar, V.; Satyanarayana, T.
    Generation of xylooligosaccharides from microwave irradiated agroresidues using recombinant thermo-alkali-stable endoxylanase of the polyextremophilic bacterium Bacillus halodurans expressed in Pichia pastoris (2015), Biores. Technol., 179, 382-389.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 2-mercaptoethanol
-
 Halalkalibacterium halodurans
3.2.1.8 dithiothreitol
-
 Halalkalibacterium halodurans

Application

EC Number Application Comment Organism
3.2.1.8 degradation agroresidues subjected to alkali and microwave irradiation for 6 min in order to expose the polysaccharide component to enzymatic hydrolysis lead to increased relase of sugars. The maximum sugar content is detected in the hydrolysate of microwave-irradiated wheat bran (6.20 mg/g substrate) followed by wheat straw (4.9 mg/g substrate) Halalkalibacterium halodurans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression in Pichia pastoris, yields a high titer of extracellular xylanase (502 U/ml) on induction with methanol Halalkalibacterium halodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cd2+ strong inhibition Halalkalibacterium halodurans
3.2.1.8 Cu2+ strong inhibition Halalkalibacterium halodurans
3.2.1.8 Hg2+ strong inhibition Halalkalibacterium halodurans
3.2.1.8 Pb2+ strong inhibition Halalkalibacterium halodurans
3.2.1.8 Sn2+ strong inhibition Halalkalibacterium halodurans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Halalkalibacterium halodurans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 45000
-
 x * 45000, SDS-PAGE Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Halalkalibacterium halodurans M4QNR9
-
-
3.2.1.8 Halalkalibacterium halodurans TSEV1 M4QNR9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 375
-
 substrate birchwood xylan, pH 9.0, 80°C Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 bagasse + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
3.2.1.8 bagasse + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?
3.2.1.8 corncob + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
3.2.1.8 corncob + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?
3.2.1.8 wheat bran + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
3.2.1.8 wheat bran + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?
3.2.1.8 wheat straw + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
3.2.1.8 wheat straw + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 45000, SDS-PAGE Halalkalibacterium halodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 80
-
-
 Halalkalibacterium halodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 80
-
 wild-type, half-life 35 min, recombinant glycosylated enzyme, half-life 45 min Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 9
-
-
 Halalkalibacterium halodurans