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Literature summary extracted from

  • Njuguna, J.T.; von Koschitzky, I.; Gerhardt, H.; Laemmerhofer, M.; Choucry, A.; Pink, M.; Schmitz-Spahnke, S.; Bakheit, M.A.; Strube, C.; Kaiser, A.
    Target evaluation of deoxyhypusine synthase from Theileria parva the neglected animal parasite and its relationship to Plasmodium (2014), Bioorg. Med. Chem., 22, 4338-4346.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.46 gene DHS, located on chromosome 2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble His-tagged enzyme in Escherichia coli strain Rosetta (DE3) Theileria parva

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.46 (4-[[2-(1H-benzimidazol-2-yl)hydrazinylidene]methyl]phenyl)(hydroxy)oxoammonium 55% inhibition Theileria parva
2.5.1.46 8-fluoro-N-[3-(morpholin-4-yl)propyl]-5H-pyrimido[5,4-b]indol-4-amine 23.6% inhibition Theileria parva
2.5.1.46 additional information inhibitor screning in an anti-malaria compound collection, a guanyl residue seems to be an important lead structure for inhibition of Theileria parva DHS enzyme Theileria parva
2.5.1.46 N-(7-chloroquinolin-4-yl)-N'-methylpropane-1,3-diamine 12.45% inhibition Theileria parva

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.46 0.37
-
 spermidine pH 9.5, 37°C, recombinant enzyme Theileria parva

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.46 41000
-
-
 Theileria parva
2.5.1.46 41260
-
-
 Theileria parva

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Theileria parva
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Theileria parva Muguga
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.46 Theileria parva Q4UDC3 gene dhs
-
2.5.1.46 Theileria parva Muguga Q4UDC3 gene dhs
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.46 recombinant soluble His-tagged enzyme from Escherichia coli strain Rosetta (DE3) Theileria parva

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.46 schizont significantly increased enzyme expression level Theileria parva
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Theileria parva [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Theileria parva Muguga [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.46 homotetramer 4 * 41000, recombinant enzyme, SDS-PAGE, 4 * 41260, about, sequence calculation Theileria parva

Synonyms

EC Number Synonyms Comment Organism
2.5.1.46 DHS
-
 Theileria parva

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.46 37
-
 assay at Theileria parva

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.46 9.5
-
 assay at Theileria parva

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.46 Theileria parva sequence calculation
-
 5.62

General Information

EC Number General Information Comment Organism
2.5.1.46 metabolism first enzyme of the hypusine pathway Theileria parva
2.5.1.46 additional information structural comparison of the three-dimensional structure of Theileria parva DHS with Homo sapiens DHS Theileria parva