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Literature summary extracted from

  • Quintas-Granados, L.I.; Carvajal Gamez, B.I.; Villalpando, J.L.; Ortega-Lopez, J.; Arroyo, R.; Azuara-Liceaga, E.; Alvarez-Sanchez, M.E.
    Bifunctional activity of deoxyhypusine synthase/hydroxylase from Trichomonas vaginalis (2016), Biochimie, 123, 37-51.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.46 gene TvDHS, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with recombinant TveIF-5A (rTveIF-5A) precursor protein and formation of a TvDHS-TveIF-5A complex Trichomonas vaginalis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.46 D314A site-directed mutagenesis, the mutant is active as deoxyhypusine synthase, but inactive as deoxyhypusine hydroxylase Trichomonas vaginalis
2.5.1.46 H313A site-directed mutagenesis, the mutant is active as deoxyhypusine synthase, but inactive as deoxyhypusine hydroxylase Trichomonas vaginalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.46 N''-guanyl-1,7-diaminoheptane GC7, a spermidine analogue Trichomonas vaginalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.46 0.0006
-
 spermidine pH 9.0, 37°C, recombinant enzyme Trichomonas vaginalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.46 43000
-
 4 * 43000, recombinant enzyme, SDS-PAGE Trichomonas vaginalis
2.5.1.46 170000
-
 recombinant enzyme, native PAGE Trichomonas vaginalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.46 additional information Trichomonas vaginalis the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29 ?
-
 ?
2.5.1.46 additional information Trichomonas vaginalis CNCD 147 the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29 ?
-
 ?
2.5.1.46 [TveIF-5A-precursor]-lysine + spermidine Trichomonas vaginalis
-
 [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [TveIF-5A-precursor]-lysine + spermidine Trichomonas vaginalis CNCD 147
-
 [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.46 Trichomonas vaginalis F1AQF9 gene TvDHS
-
2.5.1.46 Trichomonas vaginalis CNCD 147 F1AQF9 gene TvDHS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.46 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.46 additional information the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29 Trichomonas vaginalis ?
-
 ?
2.5.1.46 additional information formation of a TvDHS-TveIF-5A complex Trichomonas vaginalis ?
-
 ?
2.5.1.46 additional information the rTvDHS enzyme is bifunctional and is also involved in the hydroxylation of deoxyhypusine-containing rTveIF-5A, cf. EC 1.14.99.29 Trichomonas vaginalis CNCD 147 ?
-
 ?
2.5.1.46 additional information formation of a TvDHS-TveIF-5A complex Trichomonas vaginalis CNCD 147 ?
-
 ?
2.5.1.46 [TveIF-5A-precursor]-lysine + spermidine
-
 Trichomonas vaginalis [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [TveIF-5A-precursor]-lysine + spermidine transfer of 4-amino-2-hydroxybutyl side chain, MS/MS analysis for determination of the hypusine residue Trichomonas vaginalis [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [TveIF-5A-precursor]-lysine + spermidine
-
 Trichomonas vaginalis CNCD 147 [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [TveIF-5A-precursor]-lysine + spermidine transfer of 4-amino-2-hydroxybutyl side chain, MS/MS analysis for determination of the hypusine residue Trichomonas vaginalis CNCD 147 [TveIF-5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.46 tetramer 4 * 43000, recombinant enzyme, SDS-PAGE Trichomonas vaginalis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.46 DHS
-
 Trichomonas vaginalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.46 37
-
 assay at Trichomonas vaginalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.46 9
-
 assay at Trichomonas vaginalis