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Literature summary extracted from

  • Skopelitou, K.; Muleta, A.W.; Papageorgiou, A.C.; Chronopoulou, E.; Labrou, N.E.
    Catalytic features and crystal structure of a tau class glutathione transferase from Glycine max specifically upregulated in response to soybean mosaic virus infections (2015), Biochim. Biophys. Acta, 1854, 166-177.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21(DE3) cells Glycine max

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.18 hanging drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Bis-Tris, pH 5.6, 22% (w/v) PEG 3350 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.0286
-
 glutathione with cumene hydroperoxide as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 0.0648
-
 glutathione with fluorodifen as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 0.0662
-
 fluorodifen at pH 6.5 and 25°C Glycine max
2.5.1.18 0.0679
-
 glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 0.087
-
 glutathione with tert-butyl peroxide as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 0.252
-
 cumene hydroperoxide at pH 6.5 and 25°C Glycine max
2.5.1.18 0.28
-
 1-chloro-2,4-dinitrobenzene at pH 6.5 and 25°C Glycine max
2.5.1.18 0.853
-
 tert-butyl peroxide at pH 6.5 and 25°C Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol
-
 Glycine max 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Glycine max Q9FQE8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 1,4-butanediol diglycidyl ether-GSH-Sepharose-CL6B column chromatography Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 seedling
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Glycine max S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 cumene hydroperoxide + glutathione
-
 Glycine max ?
-
 ?
2.5.1.18 fluorodifen + glutathione
-
 Glycine max ?
-
 ?
2.5.1.18 tert-butyl peroxide + glutathione
-
 Glycine max ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GSTU10-10 isoform Glycine max
2.5.1.18 tau class glutathione transferase
-
 Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 62.4
-
 melting temperature Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.26
-
 glutathione with tert-butyl peroxide as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 0.58
-
 glutathione with cumene hydroperoxide as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 0.82
-
 glutathione with fluorodifen as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 2.65
-
 glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 6.5 and 25°C Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.5
-
-
 Glycine max

Expression

EC Number Organism Comment Expression
2.5.1.18 Glycine max isoform GSTU10-10 is specifically expressed in response to biotic stress caused by soybean mosaic virus infections up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.33
-
 tert-butyl peroxide at pH 6.5 and 25°C Glycine max
2.5.1.18 2.3
-
 cumene hydroperoxide at pH 6.5 and 25°C Glycine max
2.5.1.18 3
-
 glutathione with tert-butyl peroxide as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 9.5
-
 1-chloro-2,4-dinitrobenzene at pH 6.5 and 25°C Glycine max
2.5.1.18 12.3
-
 fluorodifen at pH 6.5 and 25°C Glycine max
2.5.1.18 12.7
-
 glutathione with fluorodifen as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 20.3
-
 glutathione with cumene hydroperoxide as cosubstrate, at pH 6.5 and 25°C Glycine max
2.5.1.18 39.2
-
 glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 6.5 and 25°C Glycine max