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Literature summary extracted from

  • Zachman-Brockmeyer, T.R.; Thoden, J.B.; Holden, H.M.
    Structures of KdnB and KdnA from Shewanella oneidensis: Key enzymes in the formation of 8-amino-3,8-dideoxy-D-manno-octulosonic acid (2016), Biochemistry, 55, 4485-4494.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.48 expressed in Escherichia coli Rosetta2-(DE3) cells Shewanella oneidensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.48 hanging drop vapor diffusion method, using 7-9% (w/v) poly(ethylene glycol) 8000, 100 mM homo-PIPES (pH 5.0), and 1.0 M tetramethylammonium chloride Shewanella oneidensis
2.6.1.109 structure of KdnA, trapped in the presence of the external aldimine with pyridoxal 5'-phosphate and glutamate, to 2.15 A resolution. KdnA adopts the classical aspartate aminotransferase fold. Residue Lys 186 forms an internal aldimine with the cofactor Shewanella oneidensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.48 3-deoxy-alpha-D-manno-octulopyranosonate + O2 Shewanella oneidensis
-
 3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
 ?
1.1.3.48 3-deoxy-alpha-D-manno-octulopyranosonate + O2 Shewanella oneidensis ATCC 700550
-
 3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.48 Shewanella oneidensis Q8EEB0
-
-
1.1.3.48 Shewanella oneidensis ATCC 700550 Q8EEB0
-
-
2.6.1.109 Shewanella oneidensis Q8EEB1
-
-
2.6.1.109 Shewanella oneidensis MR-1 / ATCC 700550 Q8EEB1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.48 Ni-NTA resin column chromatography Shewanella oneidensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.48 3-deoxy-alpha-D-manno-octulopyranosonate + O2
-
 Shewanella oneidensis 3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
 ?
1.1.3.48 3-deoxy-alpha-D-manno-octulopyranosonate + O2
-
 Shewanella oneidensis ATCC 700550 3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.48 kdnB
-
 Shewanella oneidensis
2.6.1.109 kdnA
-
 Shewanella oneidensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.48 additional information the enzyme does not require NAD(P) for activity Shewanella oneidensis
2.6.1.109 pyridoxal 5'-phosphate
-
 Shewanella oneidensis