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Literature summary extracted from

  • Syson, K.; Stevenson, C.E.; Rashid, A.M.; Saalbach, G.; Tang, M.; Tuukkanen, A.; Svergun, D.I.; Withers, S.G.; Lawson, D.M.; Bornemann, S.
    Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds alpha-maltose 1-phosphate and forms a maltosyl-enzyme intermediate (2014), Biochemistry, 53, 2494-2504.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.99.16 alpha-maltose 1-phosphate bound to a D394A mutant and beta-2-deoxy-2-fluoromaltosyl-enzyme intermediate with a E423A mutant is crystallized with 15% (w/v) polyethylene glycol 3350, 0.2 M sodium citrate, and 15% (w/v) ethylene glycol Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
2.4.99.16 D394A the maltosyl transferase activity of the mutant is more than 4 orders of magnitude lower than that of the wild type protein Streptomyces coelicolor
2.4.99.16 E423A the mutant turns over alpha-maltose 1-phosphate about 500times slower than the wild type enzyme Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.99.16 2-deoxy-2-fluoro-alpha-maltosyl fluoride after preincubation for 5 min with 1 mM substrate analogue 2-deoxy-2-fluoro-alpha-maltosyl fluoride, 70% of the normal activity with alpha-maltose 1-phosphate is lost Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.99.16 alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n Streptomyces coelicolor
-
 phosphate + [(1->4)-alpha-D-glucosyl]n+2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.16 Streptomyces coelicolor Q9L1K2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.16 2-deoxy-2-fluoro-alpha-maltosyl fluoride + maltotetraose
-
 Streptomyces coelicolor ?
-
 ?
2.4.99.16 alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n
-
 Streptomyces coelicolor phosphate + [(1->4)-alpha-D-glucosyl]n+2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.99.16 homodimer x-ray crystallography Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
2.4.99.16 alpha-maltose 1-phosphate:(1->4)-alpha-D-glucan 4-alpha-D-maltosyltransferase
-
 Streptomyces coelicolor
2.4.99.16 GlgE isoform I
-
 Streptomyces coelicolor
2.4.99.16 maltosyl transferase
-
 Streptomyces coelicolor