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Literature summary extracted from

  • Mueller, M.; Deimling, T.; Hopfner, K.P.; Witte, G.
    Structural analysis of the diadenylate cyclase reaction of DNA-integrity scanning protein A (DisA) and its inhibition by 3-dATP (2015), Biochem. J., 469, 367-374.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.85 structure in the presence of 3'-dATP and Mn2+. The metal ion is octahedrally coordinated with all six coordination positions occupied by oxygen ligands. The three phosphate groups from 3'-dATP together with Asp75 from the adjacent subunit and two water molecules are all located between 2.0 and 2.5 A from the manganese. The pre-reaction state shows a highly coordinated arrangement of the two ATP (-analogues) in almost optimal distance for the nucleophilic attack of the 3'-OH on the alpha-phosphate of the neighbouring ATP. The rate-limiting step of cyclic di-AMP synthesis by DisA is not defined by the reaction itself, but rather by the accessibility of the active site Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.85 D75N inactive Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.85 3'-dATP competitive inhibitor of the diadenylate cyclase domain, binds in the same position as ATP Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.85 42000
-
-
 Thermotoga maritima
2.7.7.85 339000
-
 gel filtration, mutant D75N Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.85 Thermotoga maritima Q9WY43
-
-
2.7.7.85 Thermotoga maritima DSM 3109 Q9WY43
-
-

Subunits

EC Number Subunits Comment Organism
2.7.7.85 octamer 8 * 42000, calculated Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.7.7.85 disA
-
 Thermotoga maritima
2.7.7.85 DNA integrity scanning protein
-
 Thermotoga maritima

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.85 0.003
-
 pH 9.5, 60°C Thermotoga maritima 3'-dATP