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Literature summary extracted from

  • Imamura, T.; Fujita, K.; Tasaki, K.; Higuchi, A.; Takahashi, H.
    Characterization of spermidine synthase and spermine synthase - The polyamine-synthetic enzymes that induce early flowering in Gentiana triflora (2015), Biochem. Biophys. Res. Commun., 463, 781-786.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 expressed in Escherichia coli BL21(DE3) cells Gentiana triflora
2.5.1.22 expressed in Escherichia coli BL21(DE3) cells Gentiana triflora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 35000
-
 x * 35000, SDS-PAGE Gentiana triflora
2.5.1.22 39000
-
 x * 39000, SDS-PAGE Gentiana triflora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl 3-(methylthio)propylamine + putrescine Gentiana triflora
-
 S-methyl-5'-thioadenosine + spermidine
-
 ?
2.5.1.22 S-adenosyl 3-(methylthio)propylamine + spermidine Gentiana triflora
-
 S-methyl-5'-thioadenosine + spermine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Gentiana triflora A0A0K2S254 cultivar SpB
-
2.5.1.22 Gentiana triflora A0A0K2S254 cultivar SpB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.22 His GraviTrap column chromatography, and gel filtration Gentiana triflora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.22 leaf
-
 Gentiana triflora
-
2.5.1.22 root
-
 Gentiana triflora
-
2.5.1.22 stem
-
 Gentiana triflora
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl 3-(methylthio)propylamine + putrescine
-
 Gentiana triflora S-methyl-5'-thioadenosine + spermidine
-
 ?
2.5.1.22 S-adenosyl 3-(methylthio)propylamine + spermidine
-
 Gentiana triflora S-methyl-5'-thioadenosine + spermine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 ? x * 35000, SDS-PAGE Gentiana triflora
2.5.1.16 ? x * 34865, calculated from amino acid sequence Gentiana triflora
2.5.1.22 ? x * 39000, SDS-PAGE Gentiana triflora
2.5.1.22 ? x * 38998, calculated from amino acid sequence Gentiana triflora

Synonyms

EC Number Synonyms Comment Organism
2.5.1.16 Spd synthase
-
 Gentiana triflora
2.5.1.16 SPDS
-
 Gentiana triflora
2.5.1.22 Spm synthase
-
 Gentiana triflora
2.5.1.22 SpmS
-
 Gentiana triflora