Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, H.; Li, Z.X.
    In vitro and in vivo characterization of a novel insect decaprenyl diphosphate synthase: a two-major step catalytic mechanism is proposed (2013), Biochem. Biophys. Res. Commun., 442, 105-111.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.86 gene AgDPPS1, two cDNAs encoding the two subunits of an aphid long-chain PDDS, designated as AgDPPS1 and AgDPPS2, AgDPPS1 encodes subunit 1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of GST-tagged subunit in Escherichia coli strain BL21(DE3) Aphis gossypii
2.5.1.86 gene AgDPPS2, two cDNAs encoding the two subunits of an aphid long-chain PDDS, designated as AgDPPS1 and AgDPPS2, AgDPPS2 encodes subunit 2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of GST-tagged subunit in Escherichia coli strain BL21(DE3) Aphis gossypii
2.5.1.91 gene AgDPPS1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strain BL21, coexpression with gene AgDPPS2 Aphis gossypii
2.5.1.91 gene AgDPPS2, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strain BL21, coexpression with gene AgDPPS1 Aphis gossypii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.86 Mg2+ required Aphis gossypii
2.5.1.91 Mg2+ required Aphis gossypii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.91 46150
-
-
 Aphis gossypii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate Aphis gossypii
-
 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
2.5.1.91 (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate Aphis gossypii
-
 7 diphosphate + all-trans-decaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.86 Aphis gossypii M9VRD0 gene AgDPPS2 encoding subunit 2
-
2.5.1.86 Aphis gossypii M9VU49 gene AgDPPS1 encoding subunit 1
-
2.5.1.91 Aphis gossypii M9VRD0 gene AgDPPS2 encoding subunit 2
-
2.5.1.91 Aphis gossypii M9VU49 gene AgDPPS1 encoding subunit 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.86 recombinant GST-tagged subunit from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, tag cleavage with factor Xa, and ultrafiltration Aphis gossypii
2.5.1.91 recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography and ultrafiltration, tag cleavage by Factor Xa, followed by again affinity chromatography and ultrafiltration Aphis gossypii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
 Aphis gossypii 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate enzyme DPPS accepts farnesyl diphosphate but not dimethylallyl diphosphate as the allylic co-substrate Aphis gossypii 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
2.5.1.86 additional information product identification by GC–MS analysis Aphis gossypii ?
-
 ?
2.5.1.91 (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
 Aphis gossypii 7 diphosphate + all-trans-decaprenyl diphosphate
-
 ?
2.5.1.91 (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate a major intermediate product (GGPP) is produced during the consecutive condensation reaction starting from FPP and IPP, leading to the formation of the final product (DPP) by adding six more IPPs. The two-major step catalytic mechanism might be a biochemical feature of insect DPPSs Aphis gossypii 7 diphosphate + all-trans-decaprenyl diphosphate
-
 ?
2.5.1.91 additional information no activity with dimethylallyl diphosphate Aphis gossypii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.86 dimer two cDNAs encoding the two subunits of an aphid long-chain PDDS, designated as AgDPPS1 and AgDPPS2 Aphis gossypii
2.5.1.91 heterodimer 1 * 46150, subunit 1/AgDPPS1 + 1 x 46740, subunit 2/AgDPPS2, sequence calculation Aphis gossypii
2.5.1.91 More the two subunits are essential for the function of enzyme DPPS, since neither AgDPPS1 nor AgDPPS2 alone show catalytic activity Aphis gossypii

Synonyms

EC Number Synonyms Comment Organism
2.5.1.86 decaprenyl diphosphate synthase 1
-
 Aphis gossypii
2.5.1.86 decaprenyl diphosphate synthase 2
-
 Aphis gossypii
2.5.1.86 DPP synthase
-
 Aphis gossypii
2.5.1.86 DPPS
-
 Aphis gossypii
2.5.1.86 polyprenyl diphosphate synthase
-
 Aphis gossypii
2.5.1.91 AgDPPS
-
 Aphis gossypii
2.5.1.91 decaprenyl diphosphate synthase 1
-
 Aphis gossypii
2.5.1.91 decaprenyl diphosphate synthase 2
-
 Aphis gossypii
2.5.1.91 decraprenyl diphosphate synthase
-
 Aphis gossypii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.91 37
-
 assay at Aphis gossypii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.86 7
-
 assay at Aphis gossypii
2.5.1.91 7
-
 assay at Aphis gossypii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.86 Aphis gossypii sequence calculation
-
 6.28
2.5.1.86 Aphis gossypii sequence calculation
-
 8.13
2.5.1.91 Aphis gossypii sequence calculation
-
 6.28
2.5.1.91 Aphis gossypii sequence calculation
-
 8.13

General Information

EC Number General Information Comment Organism
2.5.1.86 physiological function the two subunits are essential for the activity of the enzyme. The insect enzyme is a DPP synthase with a two-major step catalytic mechanism, which catalyzes the formation of decaprenyl diphosphate as the final product, with geranylgeranyl diphosphate as the major intermediate product. The insect long-chain DPPS synthesizes the side-chain of coenzyme ubiquinone 10 Aphis gossypii
2.5.1.91 evolution the enzyme contains six conserved motifs (from I to V), i.e., motif I (GKxxR), motif II (DDxxD(x)4RRG), motif III (GExxQ), motif IV (Y(x)6KT), motif V (FQLxDDxxD) and motif VI (K(x)4DxxxGxxTxPxL), occurring in all DPPS-1s examined, including insect, vertebrate, yeast and plant DPPS-1s, and bacterial DPPSs Aphis gossypii
2.5.1.91 physiological function the insect long-chain prenyl diphosphate synthase is responsible for the biosynthesis of the side-chain of ubiquinone-10, UQ-10, in Aphis gossypii Aphis gossypii