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Literature summary extracted from

  • Glisic, B.; Mihaljevic, I.; Popovic, M.; Zaja, R.; Loncar, J.; Fent, K.; Kovacevic, R.; Smital, T.
    Characterization of glutathione-S-transferases in zebrafish (Danio rerio) (2015), Aquat. Toxicol., 158, 50-62.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli Danio rerio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.004
-
 monochlorobimane isoform Gstt1a, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.05
-
 monochlorobimane isoform GstrI, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.11
-
 monochlorobimane isoform Gstm3, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.18
-
 monochlorobimane isoform Gstp2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.2
-
 Ethacrynic acid isoform Gstp1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.22
-
 monochlorobimane isoform Gstm1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.24
-
 Ethacrynic acid isoform Gstp2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.25
-
 monochlorobimane isoform Gstp1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.27
-
 glutathione isoform Gstz1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.27
-
 Dichloroacetic acid isoform Gstz1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.28
-
 1-chloro-2,4-dinitrobenzene isoform Gstp2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.32
-
 1-chloro-2,4-dinitrobenzene isoform Gstt1a, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.39
-
 1-chloro-2,4-dinitrobenzene isoform Gstp1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.5
-
 glutathione isoform Gstr1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.58
-
 glutathione isoform Gstm1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.58
-
 1-chloro-2,4-dinitrobenzene isoform Gstr1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.75
-
 glutathione isoform Gsta3, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.82
-
 glutathione isoform Gstp1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 1.08
-
 dehydroascorbate isoform Gsto2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 1.19
-
 glutathione isoform Gstp2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 1.68
-
 1-chloro-2,4-dinitrobenzene isoform Gsta3, at pH 6.5 and 25°C Danio rerio
2.5.1.18 2.05
-
 glutathione isoform Gstm3, at pH 6.5 and 25°C Danio rerio
2.5.1.18 2.24
-
 1-chloro-2,4-dinitrobenzene isoform Gstm1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 2.91
-
 glutathione isoform Gsto2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 3.69
-
 1-chloro-2,4-dinitrobenzene isoform Gstm3, at pH 6.5 and 25°C Danio rerio
2.5.1.18 4.44
-
 glutathione isoform Gstta1, at pH 6.5 and 25°C Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol
-
 Danio rerio 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Danio rerio
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 Ni-NTA column chromatography, and gel filtration Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 brain high expression of isoforms Gstz1, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
2.5.1.18 gill high expression of isoforms Gsto2, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
2.5.1.18 intestine high expression of isoforms Gsta, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
2.5.1.18 kidney high expression of isoforms Gstz1, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
2.5.1.18 liver high expression of isoforms Gstt1a, Gstz1, Gstp, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
2.5.1.18 ovary high expression of isoforms Gsto2, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-
2.5.1.18 testis high expression of isoforms Gsta, Gstp, Gstm, Gstr1, Mgst3a, and Mgst3b Danio rerio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Danio rerio S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 dehydroascorbate + glutathione substrate for isoform Gsto2 Danio rerio ?
-
 ?
2.5.1.18 dichloroacetic acid + glutathione substrate for isoform Gstz1 Danio rerio ?
-
 ?
2.5.1.18 ethacrynic acid + glutathione substrate for isoforms Gstp1 and Gstp2 Danio rerio ?
-
 ?
2.5.1.18 monochlorobimane + glutathione
-
 Danio rerio ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione-S-transferas
-
 Danio rerio
2.5.1.18 GST
-
 Danio rerio
2.5.1.18 Gsta isoform Danio rerio
2.5.1.18 Gstm isoform Danio rerio
2.5.1.18 GSTO2 isoform Danio rerio
2.5.1.18 GSTP isoform Danio rerio
2.5.1.18 Gstr1 isoform Danio rerio
2.5.1.18 Gstt1a isoform Danio rerio
2.5.1.18 Gstz1 isoform Danio rerio
2.5.1.18 Mgst3a isoform Danio rerio
2.5.1.18 Mgst3b isoform Danio rerio

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.002
-
 Dichloroacetic acid isoform Gstz1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 0.005
-
 glutathione isoform Gstz1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 1.23
-
 Ethacrynic acid isoform Gstp2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 2.24
-
 Ethacrynic acid isoform Gstp1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 8.69
-
 glutathione isoform Gsto2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 11.2
-
 glutathione isoform Gstta1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 11.9
-
 dehydroascorbate isoform Gsto2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 14.1
-
 glutathione isoform Gstp2, at pH 6.5 and 25°C Danio rerio
2.5.1.18 16.6
-
 glutathione isoform Gsta3, at pH 6.5 and 25°C Danio rerio
2.5.1.18 20.8
-
 glutathione isoform Gstr1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 22.7
-
 glutathione isoform Gstm1, at pH 6.5 and 25°C Danio rerio
2.5.1.18 57.2
-
 glutathione isoform Gstm3, at pH 6.5 and 25°C Danio rerio
2.5.1.18 129
-
 glutathione isoform Gstp1, at pH 6.5 and 25°C Danio rerio