EC Number | Application | Comment | Organism |
---|---|---|---|
2.5.1.109 | synthesis | regularly C7-prenylated cyclic dipeptides are successfully obtained by using the member of the DMATS family, CdpC7PT from Aspergillus terreus | Aspergillus terreus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.109 | gene ATEG_04218 or cdpC7PT, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain M15 | Aspergillus terreus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.109 | additional information | - |
additional information | Michaelis-Menten kinetics, for the tryptophan-containing cyclic dipeptides, KM values between 0.239 and 0.747 mM and turnover numbers in the range of 0.009-0.049/s are determined | Aspergillus terreus | |
2.5.1.109 | 0.0785 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Aspergillus terreus | |
2.5.1.109 | 0.204 | - |
(S)-benzodiazepinedione | pH 7.5, 37°C | Aspergillus terreus | |
2.5.1.109 | 1.411 | - |
cyclo-L-Tyr-L-Tyr | pH 7.5, 37°C | Aspergillus terreus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.109 | Ca2+ | activates 58% at 5 mM | Aspergillus terreus | |
2.5.1.109 | Mg2+ | activates 20% at 5 mM | Aspergillus terreus | |
2.5.1.109 | Mn2+ | activates 31% at 5 mM | Aspergillus terreus | |
2.5.1.109 | additional information | no significant effect by 5 mM EDTA. The enzyme reaction of CdpC7PT is independent of metal ions, but can be enhanced by Ca2+, Mn2+, and Mg2+ | Aspergillus terreus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.109 | 62000 | - |
recombinant His6-tagged enzyme, gel filtration | Aspergillus terreus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Trp | Aspergillus terreus | attachment of the prenyl moiety to C-7 of the indole ring | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-Xaa | Aspergillus oryzae | - |
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-Xaa | - |
? | |
2.5.1.109 | additional information | Aspergillus terreus | for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products | ? | - |
? | |
2.5.1.109 | additional information | Aspergillus terreus DSM 1958 | for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.109 | Aspergillus oryzae | - |
gene cdpC7PT | - |
2.5.1.109 | Aspergillus terreus | Q0CQ16 | gene ATEG_04218 or cdpC7PT | - |
2.5.1.109 | Aspergillus terreus DSM 1958 | Q0CQ16 | gene ATEG_04218 or cdpC7PT | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.109 | recombinant His6-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography | Aspergillus terreus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.109 | mycelium | - |
Aspergillus oryzae | - |
2.5.1.109 | mycelium | - |
Aspergillus terreus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.109 | dimethylallyl diphosphate + (R)-benzodiazepinedione | low activity, (R)-benzodiazepinedione consists of a tryptophanyl and an anthranilyl moiety | Aspergillus terreus | diphosphate + ? | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + (S)-benzodiazepinedione | best substrate, (S)-benzodiazepinedione consists of a tryptophanyl and an anthranilyl moiety | Aspergillus terreus | diphosphate + ? | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + benzodiazepinedione | best substrate, active only with benzodiazepinedione consisting of a D-tryptophanyl and an anthranilyl moiety, poor activity with the L-Tryp isomer | Aspergillus terreus | diphosphate + ? | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-D-Trp-D-Pro | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-D-Pro | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-D-Trp-D-Pro | low activity | Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-D-Pro | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-D-Trp-L-Ala | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-L-Ala | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-D-Trp-L-Pro | low activity | Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-L-Pro | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-D-Trp-L-Trp | low activity | Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-L-Trp | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-D-Ala | low activity | Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-D-Ala | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-D-Pro | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-D-Pro | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-Gly | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-Gly | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Ala | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Ala | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Leu | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Leu | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Pro | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Pro | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Trp | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Trp | higher activity | Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Trp | attachment of the prenyl moiety to C-7 of the indole ring | Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Trp | - |
Aspergillus terreus DSM 1958 | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Tyr | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr | higher activity | Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Tyr | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Trp-Xaa | - |
Aspergillus oryzae | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-Xaa | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-L-Tyr-L-Tyr | - |
Aspergillus terreus | diphosphate + 4-O-(3-methylbut-2-enyl)-cyclo-L-Tyr-L-Tyr | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + cyclo-LTrp-L-Phe | - |
Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Phe | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + L-tryptophan | reaction of 7-DMATS, EC 2.5.1.80, low activity | Aspergillus terreus | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.109 | dimethylallyl diphosphate + L-tryptophan | reaction of 7-DMATS, EC 2.5.1.80, low activity | Aspergillus terreus DSM 1958 | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.109 | additional information | for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products | Aspergillus terreus | ? | - |
? | |
2.5.1.109 | additional information | the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring, no activity with cyclo-L-Trp-L-Pro, cyclo-L-Trp-D-Pro, cyclo-D-Trp-L-Pro, cyclo-D-Trp-D-Pro, and cyclo-L-Trp-Gly | Aspergillus terreus | ? | - |
? | |
2.5.1.109 | additional information | the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring. It also accepts cyclo-L-Tyr-L-Tyr as substrate and catalyzes an O-prenylation at the tyrosyl residue of the dipeptide cyclo-L-Trp-L-Tyr. Substrate specificity, overview. Cyclo-L-Tyr-L-Ser, cyclo-L-Tyr-L-Pro, cyclo-L-Tyr-Gly, and L-tyrosine are poor substrates. CdpC7PT shows a high affinity to its prenyl donor dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
2.5.1.109 | additional information | the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring. The enzyme showsma relatively narrow substrate specificity | Aspergillus oryzae | ? | - |
? | |
2.5.1.109 | additional information | for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products | Aspergillus terreus DSM 1958 | ? | - |
? | |
2.5.1.109 | additional information | the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring, no activity with cyclo-L-Trp-L-Pro, cyclo-L-Trp-D-Pro, cyclo-D-Trp-L-Pro, cyclo-D-Trp-D-Pro, and cyclo-L-Trp-Gly | Aspergillus terreus DSM 1958 | ? | - |
? | |
2.5.1.109 | additional information | the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring. It also accepts cyclo-L-Tyr-L-Tyr as substrate and catalyzes an O-prenylation at the tyrosyl residue of the dipeptide cyclo-L-Trp-L-Tyr. Substrate specificity, overview. Cyclo-L-Tyr-L-Ser, cyclo-L-Tyr-L-Pro, cyclo-L-Tyr-Gly, and L-tyrosine are poor substrates. CdpC7PT shows a high affinity to its prenyl donor dimethylallyl diphosphate | Aspergillus terreus DSM 1958 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.109 | CdpC7PT | - |
Aspergillus oryzae |
2.5.1.109 | CdpC7PT | - |
Aspergillus terreus |
2.5.1.109 | cyclic dipeptide C7-prenyltransferase | - |
Aspergillus oryzae |
2.5.1.109 | cyclic dipeptide C7-prenyltransferase | - |
Aspergillus terreus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.109 | 37 | - |
assay at | Aspergillus terreus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.109 | 0.012 | - |
cyclo-L-Tyr-L-Tyr | pH 7.5, 37°C | Aspergillus terreus | |
2.5.1.109 | 0.067 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Aspergillus terreus | |
2.5.1.109 | 0.125 | - |
(S)-benzodiazepinedione | pH 7.5, 37°C | Aspergillus terreus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.109 | 7.5 | - |
assay at | Aspergillus terreus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.109 | evolution | the enzyme belongs to the DMATS superfamily | Aspergillus oryzae |
2.5.1.109 | evolution | the enzyme belongs to the DMATS superfamily | Aspergillus terreus |