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Literature summary extracted from

  • Chefetz, B.; Chen, Y.; Hadar, Y.
    Purification and characterization of laccase from Chaetomium thermophilium and its role in humification (1998), Appl. Environ. Microbiol., 64, 3175-3179.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 EDTA 5 mM, 67% inhibition. 10 mM, 95% inhibition Thermochaetoides thermophila
1.10.3.2 additional information strongly inhibited by Cu-chelating agents Thermochaetoides thermophila
1.10.3.2 Sodium azide 0.05 mM, 84% inhibition Thermochaetoides thermophila
1.10.3.2 thioglycolic acid 0.1 mM, 91% inhibition Thermochaetoides thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.034
-
 syringaldazine pH 6.0, 50°C Thermochaetoides thermophila
1.10.3.2 0.036
-
 hydroxyquinone pH 6.0, 50°C Thermochaetoides thermophila
1.10.3.2 0.096
-
 2,6-dimethoxyphenol pH 6.0, 50°C Thermochaetoides thermophila
1.10.3.2 0.1
-
 L-3,4-dihydroxyphenylalanine pH 6.0, 50°C Thermochaetoides thermophila
1.10.3.2 0.13
-
 gallic acid pH 6.0, 50°C Thermochaetoides thermophila
1.10.3.2 0.15
-
 vanillic acid pH 6.0, 50°C Thermochaetoides thermophila
1.10.3.2 0.19
-
 2,2'-azinobis(3-ethylbenz-thiazoline-6-sulfonic acid) pH 6.0, 50°C Thermochaetoides thermophila
1.10.3.2 0.27
-
 ferulic acid pH 6.0, 50°C Thermochaetoides thermophila
1.10.3.2 0.4
-
 guaiacol pH 6.0, 50°C Thermochaetoides thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 77000
-
 x * 77000, SDS-PAGE Thermochaetoides thermophila

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Thermochaetoides thermophila
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein
-
 Thermochaetoides thermophila

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Thermochaetoides thermophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 37
-
 pH 6.0, 50°C Thermochaetoides thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenz-thiazoline-6-sulfonic acid) + O2
-
 Thermochaetoides thermophila ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Thermochaetoides thermophila ?
-
 ?
1.10.3.2 ferulic acid + O2
-
 Thermochaetoides thermophila ?
-
 ?
1.10.3.2 gallic acid + O2
-
 Thermochaetoides thermophila ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Thermochaetoides thermophila ?
-
 ?
1.10.3.2 hydroxyquinone + O2
-
 Thermochaetoides thermophila ?
-
 ?
1.10.3.2 L-3,4-dihydroxyphenylalanine + O2 i.e. L-dopa Thermochaetoides thermophila ?
-
 ?
1.10.3.2 additional information no activity with tyrosine Thermochaetoides thermophila ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Thermochaetoides thermophila ?
-
 ?
1.10.3.2 vanillic acid + O2
-
 Thermochaetoides thermophila ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 77000, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50 60
-
 Thermochaetoides thermophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 40 70 40°C: about 50% of maximal activity, 70°C: about 90% of maximal activity Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 half-life: 24 h Thermochaetoides thermophila
1.10.3.2 50
-
 half-life: 12 h Thermochaetoides thermophila
1.10.3.2 70
-
 1 h, stable Thermochaetoides thermophila
1.10.3.2 80
-
 half-life: 10 min Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 6
-
 oxidation of syringaldazine Thermochaetoides thermophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 5 8 pH 5.0: about 20% of maximal activity, pH 8.0: about 65% of maximal activity. The enzyme is inactive at pH 4.0 and at pH 9.0 Thermochaetoides thermophila

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 5 10 1 h, stable Thermochaetoides thermophila

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Thermochaetoides thermophila isoelectric focusing
-
 5.1