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Literature summary extracted from

  • Mitachi, K.; Siricilla, S.; Yang, D.; Kong, Y.; Skorupinska-Tudek, K.; Swiezewska, E.; Franzblau, S.G.; Kurosu, M.
    Fluorescence-based assay for polyprenyl phosphate-GlcNAc-1-phosphate transferase (WecA) and identification of novel antimycobacterial WecA inhibitors (2016), Anal. Biochem., 512, 78-90.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.8.35 drug development the enzyme is a target for development of drugs Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.35 gene wecA, recombinant overexpression in Escherichia coli strain BL21 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.35 DMSO 50% inhibition at 10% v/v, no inhibition at 2.86% v/v Escherichia coli
2.7.8.35 DMSO 50% inhibition at 10% v/v, no inhibition at 2.86% v/v Mycolicibacterium smegmatis
2.7.8.35 additional information synthesis and evaluation of a series of capuramycin analogues as WecA enzyme inhibitors. No inhibition by capuramycin, UT-01309, and SQ641 Escherichia coli
2.7.8.35 additional information synthesis and evaluation of a series of capuramycin analogues as WecA enzyme inhibitors. No inhibition by capuramycin, UT-01309, and SQ641 Mycolicibacterium smegmatis
2.7.8.35 tunicamycin complete inhibition at 0.02 mg/ml, 36% at 20 ng/ml Escherichia coli
2.7.8.35 tunicamycin
-
 Mycobacterium tuberculosis
2.7.8.35 tunicamycin
-
 Mycolicibacterium smegmatis
2.7.8.35 UT-01320 complete inhibition at 0.02 mg/ml, 42% at 20 ng/ml Escherichia coli
2.7.8.35 UT-01320 a selective WecA inhibitor that kills both replicating and non-replicating Mycobacterium tuberculosis cells at 0.0015-0.00258 mg/ml , respectively Mycobacterium tuberculosis
2.7.8.35 UT-01320
-
 Mycolicibacterium smegmatis
2.7.8.35 UT-03325 55% inhibition at 0.02 mg/ml, 30% at 20 ng/ml Escherichia coli
2.7.8.35 UT-03325
-
 Mycolicibacterium smegmatis
2.7.8.35 UT-03328 60.5% inhibition at 0.02 mg/ml, no inhibition at 20 ng/ml Escherichia coli
2.7.8.35 UT-03328
-
 Mycolicibacterium smegmatis
2.7.8.35 UT-03347 80.5% inhibition at 0.02 mg/ml, 11% at 20 ng/ml Escherichia coli
2.7.8.35 UT-03347
-
 Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.35 additional information
-
 additional information Michaelis-Menten enzyme kinetics Escherichia coli
2.7.8.35 additional information
-
 additional information Michaelis-Menten enzyme kinetics Mycobacterium tuberculosis
2.7.8.35 additional information
-
 additional information Michaelis-Menten enzyme kinetics Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.35 Mg2+ required Escherichia coli
2.7.8.35 Mg2+ required Mycobacterium tuberculosis
2.7.8.35 Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate Escherichia coli
-
 UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate Mycobacterium tuberculosis
-
 UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate Mycolicibacterium smegmatis
-
 UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate Mycobacterium tuberculosis H37Rv
-
 UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.35 Escherichia coli P0AC78 gene wecA
-
2.7.8.35 Mycobacterium tuberculosis P9WMW5 gene wecA
-
2.7.8.35 Mycobacterium tuberculosis H37Rv P9WMW5 gene wecA
-
2.7.8.35 Mycolicibacterium smegmatis A0R211 gene wecA
-
2.7.8.35 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0R211 gene wecA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.35 preparation of membrane fraction Mycobacterium tuberculosis
2.7.8.35 preparation of membrane fraction Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.35 additional information specificity of WecA against truncated prenyl phosphates, overview Mycobacterium tuberculosis ?
-
 ?
2.7.8.35 additional information specificity of WecA against truncated prenyl phosphates, overview. Decaprenyl phosphate and undecaprenyl phosphate are equally effective, no activity with substrates that have shorter prenyl chains Escherichia coli ?
-
 ?
2.7.8.35 additional information specificity of WecA against truncated prenyl phosphates, overview. Decaprenyl phosphate and undecaprenyl phosphate are equally effective, no activity with substrates that have shorter prenyl chains Mycolicibacterium smegmatis ?
-
 ?
2.7.8.35 additional information specificity of WecA against truncated prenyl phosphates, overview. Decaprenyl phosphate and undecaprenyl phosphate are equally effective, no activity with substrates that have shorter prenyl chains Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?
2.7.8.35 additional information specificity of WecA against truncated prenyl phosphates, overview Mycobacterium tuberculosis H37Rv ?
-
 ?
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate
-
 Escherichia coli UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate
-
 Mycobacterium tuberculosis UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate
-
 Mycolicibacterium smegmatis UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
2.7.8.35 UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate
-
 Mycobacterium tuberculosis H37Rv UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.35 polyprenyl phosphate-GlcNAc-1-phosphate transferase
-
 Escherichia coli
2.7.8.35 polyprenyl phosphate-GlcNAc-1-phosphate transferase
-
 Mycobacterium tuberculosis
2.7.8.35 polyprenyl phosphate-GlcNAc-1-phosphate transferase
-
 Mycolicibacterium smegmatis
2.7.8.35 WecA
-
 Escherichia coli
2.7.8.35 WecA
-
 Mycobacterium tuberculosis
2.7.8.35 WecA
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.35 37
-
 assay at Escherichia coli
2.7.8.35 37
-
 assay at Mycobacterium tuberculosis
2.7.8.35 37
-
 assay at Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.35 8
-
 assay at Escherichia coli
2.7.8.35 8
-
 assay at Mycobacterium tuberculosis
2.7.8.35 8
-
 assay at Mycolicibacterium smegmatis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.8.35 additional information
-
 IC50 for tunicamycin is 120 ng/ml at pH 8.0 37°C Escherichia coli additional information
2.7.8.35 additional information
-
 IC50 for tunicamycin is 590 ng/ml at pH 8.0 37°C Mycolicibacterium smegmatis additional information

General Information

EC Number General Information Comment Organism
2.7.8.35 malfunction inhibition of WecA will block the entire biosynthesis of essential cell wall components of Mtb in both replicating and non-replicating states Mycobacterium tuberculosis
2.7.8.35 physiological function WecA catalyzes the transformation from UDP-N-acetyl-alpha-D-glucosamine to N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol the first membrane-anchored glycophospholipid that is responsible for the biosynthesis of mycolylarabinogalactan Mycobacterium tuberculosis