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Literature summary extracted from

  • van der Straat, L.; Tamayo-Ramos, J.; Schonewille, T.; de Graaff, L.
    Overexpression of a modified 6-phosphofructo-1-kinase results in an increased itaconic acid productivity in Aspergillus niger (2013), AMB Express, 3, 1-19.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.7.1.11 synthesis expression of a modified 6-phosphofructo-1-kinase in a citrate producing Aspergillus niger strain in combination with cis-aconitate decarboxylase CadA from Aspergillus terreus to study the effect on the production of itaconic acid. The combined expression of pfkA and cadA results in increased citrate levels, but does not show increased itaconic acid levels. The combined expression of pfkA with the itaconic acid biosynthetic cluster, consisiting of cis-aconitate decarboxylase cadA, a putative mitochondrial transporter mttA and a putative plasmamembrane transporter mfsA, results in significantly increased itaconic acid production at earlier time points and significant increase in itaconic acid productivity. The maximum itaconic acid productivity reached is 0.15 g per l and h Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11
-
Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Aspergillus niger U4MZ33
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 PFKA
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Aspergillus niger