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Literature summary extracted from

  • Schmidberger, J.W.; Schnell, R.; Schneider, G.
    Structural characterization of substrate and inhibitor binding to farnesyl pyrophosphate synthase from Pseudomonas aeruginosa (2015), Acta Crystallogr. Sect. D, 71, 721-731.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.10 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.10 in complex with natural substrates and inhibitors, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M NaF, 0.1 M bis-Tris propane pH 6.5 Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.10 ibandronate
-
 Pseudomonas aeruginosa
2.5.1.10 zoledronate
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.10 Mg2+ contains Mg2+ Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.10 34018
-
 ESI-MS Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Pseudomonas aeruginosa
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate Pseudomonas aeruginosa
-
 diphosphate + geranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Pseudomonas aeruginosa Q9HWY4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10 Ni-NTA resin column chromatography and Superdex 200 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Pseudomonas aeruginosa diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate
-
 Pseudomonas aeruginosa diphosphate + geranyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.10 ? x * 34018, calculated from amino acid sequence Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 farnesyl pyrophosphate synthase
-
 Pseudomonas aeruginosa
2.5.1.10 FPPS
-
 Pseudomonas aeruginosa
2.5.1.10 PA4043
-
 Pseudomonas aeruginosa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.10 0.0027
-
 at pH 8.0 and 22°C Pseudomonas aeruginosa ibandronate
2.5.1.10 0.0152
-
 at pH 8.0 and 22°C Pseudomonas aeruginosa zoledronate