EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.7.47 | to 2.5 A resolution. AadA consists of a nucleotidyltransferase domain and an alpha-helical bundle domain. AadA crystallizes as a monomer. ATP binding has to occur before binding of the aminoglycoside substrate, and ATP binding repositions the two domains for aminoglycoside binding in the interdomain cleft | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.47 | D182A | mutation reduces the MIC of streptomycin and spectinomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.47 | D182N | mutation reduces the MIC of streptomycin and spectinomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.47 | E87A | mutation reduces the MIC of streptomycin and spectinomycin to that of an AadA null strain | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.47 | E87Q | mutation reduces the MIC of streptomycin and spectinomycin to that of an AadA null strain | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.47 | K205A | mutation reduces the MIC of streptomycin and spectinomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.47 | R192A | mutation reduces the MIC of streptomycin and spectinomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.47 | W112A | mutation reduces the MIC of spectinomycin to that of an AadA null strain and reduces the MIC of streptomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.7.47 | W112F | mutation reduces the MIC of spectinomycin to that of an AadA null strain and reduces the MIC of streptomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.47 | Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZPX9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.47 | ATP + spectinomycin | - |
Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + 9-adenylylspectinomycin | - |
? | |
2.7.7.47 | ATP + streptomycin | - |
Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + 3''-adenylylstreptomycin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.47 | monomer | small-angle X-ray scattering | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.47 | aadA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.7.47 | Salmonella enterica subsp. enterica serovar Typhimurium | calculated | - |
5 |