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Literature summary extracted from

  • Aripirala, S.; Gonzalez-Pacanowska, D.; Oldfield, E.; Kaiser, M.; Amzel, L.M.; Gabelli, S.B.
    Structural and thermodynamic basis of the inhibition of Leishmania major farnesyl diphosphate synthase by nitrogen-containing bisphosphonates (2014), Acta Crystallogr. Sect. D, 70, 802-810.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.10 in complex with isopentenyl diphosphate and inhibitors, hanging drop vapor diffusion method, using 15-25% (w/v) PEG 3350, 0.1-0.2 M calcium acetate, 0.1 M MES sodium salt pH 6.5 Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.10 1-(2-hydroxy-2,2-diphosphonoethyl)-3-phenylpyridinium
-
Leishmania major
2.5.1.10 3-butyl-1-(2,2-diphosphonoethyl)pyridinium
-
Leishmania major
2.5.1.10 3-fluoro-1-(2-hydroxy-2,2-diphosphonoethyl)-pyridinium
-
Leishmania major
2.5.1.10 zoledronate
-
Leishmania major

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.10 Mg2+ contains Mg2+ Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Leishmania major
-
diphosphate + (2E,6E)-farnesyl diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Leishmania major
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10 HisTrap Ni2+-chelate column chromatography Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
Leishmania major diphosphate + (2E,6E)-farnesyl diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.10 homodimer
-
Leishmania major

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 farnesyl pyrophosphate synthase
-
Leishmania major
2.5.1.10 FPPS
-
Leishmania major

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.10 0.000001
-
1-(2-hydroxy-2,2-diphosphonoethyl)-3-phenylpyridinium pH and temperature not specified in the publication Leishmania major
2.5.1.10 0.000011
-
zoledronate pH and temperature not specified in the publication Leishmania major
2.5.1.10 0.00005
-
3-fluoro-1-(2-hydroxy-2,2-diphosphonoethyl)-pyridinium pH and temperature not specified in the publication Leishmania major