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Literature summary extracted from

  • Shankar, M.; Wilbanks, S.M.; Nakatani, Y.; Monk, B.C.; Tyndall, J.D.
    Catalysis product captured in lumazine synthase from the fungal pathogen Candida glabrata (2013), Acta Crystallogr. Sect. D, 69, 1580-1586.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.78 drug development the enzyme is a potential drug target for the development of broad-spectrum antifungal drugs Nakaseomyces glabratus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.78 gene RIB4A, recombinant expression of His-tagged enzyme in Escherichia coli Nakaseomyces glabratus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.78 purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 200 nl 8.4 mg/ml protein in 100 mM potassium phosphate, pH 7.5, and 150 mM NaCl, with 200 nl of reservoir solution containing 1.6 M ammonium sulfate, 100 mM sodium acetate pH 5.5, 100 mM sodium chloride, and equilibration against 0.1 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.24 A resolution Nakaseomyces glabratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.78 97770
-
 MALDI-TOF mass spectromety Nakaseomyces glabratus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.78 Nakaseomyces glabratus Q6FXA8 gene RIB4A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.78 gene RIB4A, recombinant His-tagged enzyme from Escherichia coli by nickel affinityy chromatography, gel filtration and ultrafiltration Nakaseomyces glabratus

Subunits

EC Number Subunits Comment Organism
2.5.1.78 decamer 10 * 19548, His-tagged enzyme, (alpha5)2, a dimer of homopentamers with one in five subunits containing a product molecule from the catalytic reaction Nakaseomyces glabratus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.78 6,7-dimethyl-8-(d-ribityl)lumazine synthase
-
 Nakaseomyces glabratus
2.5.1.78 lumazine synthase
-
 Nakaseomyces glabratus

General Information

EC Number General Information Comment Organism
2.5.1.78 physiological function the enzyme 6,7-dimethyl-8-(d-ribityl)lumazine synthase is part of the riboflavin biosynthesis pathway essential to fungi and bacteria Nakaseomyces glabratus