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Literature summary extracted from

  • Jiang, W.; Xia, B.; Huang, J.; Liu, Z.
    Characterization of a serine hydroxymethyltransferase for L-serine enzymatic production from Pseudomonas plecoglossicida (2013), World J. Microbiol. Biotechnol., 29, 2067-2076.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 gene glyA, DNA and amino acid sequence determination and analysis, phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) Pseudomonas plecoglossicida

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 Co2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
2.1.2.1 CTAB
-
 Pseudomonas plecoglossicida
2.1.2.1 Cu2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
2.1.2.1 Fe2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
2.1.2.1 Hg2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
2.1.2.1 Mn2+ strong inhibition at 1 mM Pseudomonas plecoglossicida
2.1.2.1 SDS
-
 Pseudomonas plecoglossicida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.1 additional information K+, Na+, NH4+, Mg2+, Ca2+, Zn2+ and Pb2+ show no significant effect on enzyme activity Pseudomonas plecoglossicida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 44500
-
 x * 44500, about, sequence calculation, x * 60000, recombinant GST-tagged enzyme, SDS-PAGE Pseudomonas plecoglossicida
2.1.2.1 60000
-
 x * 44500, about, sequence calculation, x * 60000, recombinant GST-tagged enzyme, SDS-PAGE Pseudomonas plecoglossicida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Pseudomonas plecoglossicida
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Pseudomonas plecoglossicida MCCC 1K00022
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine Pseudomonas plecoglossicida
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine Pseudomonas plecoglossicida MCCC 1K00022
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Pseudomonas plecoglossicida L7Y8B0 gene glyA
-
2.1.2.1 Pseudomonas plecoglossicida MCCC 1K00022 L7Y8B0 gene glyA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Pseudomonas plecoglossicida tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Pseudomonas plecoglossicida MCCC 1K00022 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Pseudomonas plecoglossicida 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Pseudomonas plecoglossicida MCCC 1K00022 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 ? x * 44500, about, sequence calculation, x * 60000, recombinant GST-tagged enzyme, SDS-PAGE Pseudomonas plecoglossicida

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Pseudomonas plecoglossicida
2.1.2.1 SHMT
-
 Pseudomonas plecoglossicida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 40
-
-
 Pseudomonas plecoglossicida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.2.1 5 45 60% of maximal activity at 30°C and 45°C, rapid loss of activity above Pseudomonas plecoglossicida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 8
-
-
 Pseudomonas plecoglossicida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.1 5.8 9 almost inactive at pH 5.8, less than 10% of maximal activity at pH 6.0, maximal activity at pH 8.0, 80% of maximal activity at pH 7.5 and pH 8.5, inactive at pH 9.5 Pseudomonas plecoglossicida

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 5,10-methylenetetrahydrofolate
-
 Pseudomonas plecoglossicida
2.1.2.1 pyridoxal 5'-phosphate dependent on Pseudomonas plecoglossicida
2.1.2.1 tetrahydrofolate
-
 Pseudomonas plecoglossicida