Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ryan, K.L.; Moore, C.T.; Panaccione, D.G.
    Partial reconstruction of the ergot alkaloid pathway by heterologous gene expression in Aspergillus nidulans (2013), Toxins, 5, 445-455.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.261 gene easF, a fragment of genomic DNA containing dmaW, easF, easE, and easC is amplified from the human-pathogenic, ergot alkaloid-producing fungus Aspergillus fumigatus and transformed into Aspergillus nidulans strain FGSC A767, a model fungus that does not contain any of the ergot alkaloid synthesis genes. Transformed Aspergillus nidulans strains produces chanoclavine-I and an earlier pathway intermediate Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan Aspergillus fumigatus
-
 S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.261 Aspergillus fumigatus Q4WZ60 gene easF
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
 Aspergillus fumigatus S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
 ?
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan easF encodes a methyltransferase that N-methylates DMAT to form the second intermediate, N-Me-DMAT Aspergillus fumigatus S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.261 S-adenosyl-L-methionine
-
 Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.1.1.261 metabolism the enzyme catalyzes the second step in the biosynthesis of ergot alkaloids, five steps of ergot alkaloid synthesis leading to the formation of chanoclavine-I, overview Aspergillus fumigatus