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Literature summary extracted from

  • Mittelstaedt, G.; Moggre, G.J.; Panjikar, S.; Nazmi, A.R.; Parker, E.J.
    Campylobacter jejuni adenosine triphosphate phosphoribosyltransferase is an active hexamer which is allosterically controlled by the twisting of a regulatory tail (2016), Protein Sci., 25, 1492-1506.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.17 expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.17 in complex with ATP, L-histidine, or L-histidine/AMP, hanging drop vapor diffusion method, using 0.1M sodium acetate, 0.1 M MgCl2, 13-15% (w/v) PEG 4000, pH 5.5 (ATP), or 0.1 M BTP, 0.2 M KSCN, 13-14% (w/v) PEG 3350, pH 6.5 (His), or 0.1 M Tris, 0.1 M MgCl2, 13-15% (w/v) PEG 4000, pH 7.5 (L-His/AMP) Campylobacter jejuni

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 AMP competitive inhibition Campylobacter jejuni
2.4.2.17 L-histidine allosterical inhibition Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.17 0.015
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C Campylobacter jejuni
2.4.2.17 0.097
-
 ATP at pH 8.5 and 25°C Campylobacter jejuni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.17 Mg2+ essential Campylobacter jejuni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.17 184000
-
 gel filtration Campylobacter jejuni
2.4.2.17 188000
-
 SEC-coupled static light scattering Campylobacter jejuni
2.4.2.17 202000
-
 calculated from amino acid sequence Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Campylobacter jejuni
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Campylobacter jejuni NCTC 11168
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Campylobacter jejuni
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Campylobacter jejuni NCTC 11168
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Campylobacter jejuni Q9PM78
-
-
2.4.2.17 Campylobacter jejuni NCTC 11168 Q9PM78
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.17 HiTrap Talon column chromatography and Superdex 200 gel filtration Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Campylobacter jejuni ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Campylobacter jejuni NCTC 11168 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Campylobacter jejuni 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Campylobacter jejuni NCTC 11168 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.17 hexamer
-
 Campylobacter jejuni

Synonyms

EC Number Synonyms Comment Organism
2.4.2.17 adenosine triphosphate phosphoribosyltransferase
-
 Campylobacter jejuni
2.4.2.17 ATP-PRT
-
 Campylobacter jejuni
2.4.2.17 HisGL
-
 Campylobacter jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.17 1.8
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C Campylobacter jejuni

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.17 0.04
-
 L-histidine with respect to 5-phospho-alpha-D-ribose 1-diphosphate, at pH 8.5 and 25°C Campylobacter jejuni
2.4.2.17 0.044
-
 L-histidine with respect to ATP, at pH 8.5 and 25°C Campylobacter jejuni
2.4.2.17 0.356
-
 AMP with respect to ATP, at pH 8.5 and 25°C Campylobacter jejuni
2.4.2.17 0.382
-
 AMP with respect to 5-phospho-alpha-D-ribose 1-diphosphate, at pH 8.5 and 25°C Campylobacter jejuni

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.17 19
-
 ATP at pH 8.5 and 25°C Campylobacter jejuni
2.4.2.17 120
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C Campylobacter jejuni