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Literature summary extracted from

  • Barbier, T.; Collard, F.; Zuniga-Ripa, A.; Moriyon, I.; Godard, T.; Becker, J.; Wittmann, C.; Van Schaftingen, E.; Letesson, J.J.
    Erythritol feeds the pentose phosphate pathway via three new isomerases leading to D-erythrose-4-phosphate in Brucella (2014), Proc. Natl. Acad. Sci. USA, 111, 17815-17820.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B58 expressed in Escherichia coli BL21pLysS cells Brucella sp.
5.1.3.38 eryC is inserted into a pET15b vector, which allows expression of N-terminal His-tagged proteins in Escherichia coli Bl21pLysS Brucella abortus
5.3.1.33 tpiA2 is inserted into a pET15b vector, which allows expression of N-terminal His-tagged proteins in Escherichia coli Bl21pLysS Brucella abortus
5.3.1.34 rpiB is inserted into a pET15b vector, which allows expression of N-terminal His-tagged proteins in Escherichia coli Bl21pLysS Brucella abortus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.38 additional information metal-dependent enzyme Brucella abortus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B58 L-erythritol 4-phosphate + FAD Brucella sp.
-
 L-3-tetrulose 4-phosphate + FADH2
-
 ?
1.1.1.B58 L-erythritol 4-phosphate + NAD+ Brucella sp.
-
 L-3-tetrulose 4-phosphate + NADH + H+
-
 ?
5.1.3.38 D-erythrulose 1-phosphate Brucella abortus the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues L-erythrulose 1-phosphate
-
 ?
5.1.3.38 D-erythrulose 1-phosphate Brucella abortus 2308 the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues L-erythrulose 1-phosphate
-
 ?
5.3.1.33 L-erythrulose 1-phosphate Brucella abortus the enzyme participates in erythritol catabolism D-erythrulose 4-phosphate
-
 ?
5.3.1.33 L-erythrulose 1-phosphate Brucella abortus 2308 the enzyme participates in erythritol catabolism D-erythrulose 4-phosphate
-
 ?
5.3.1.34 D-erythrulose 4-phosphate Brucella abortus the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues D-erythrose 4-phosphate
-
 r
5.3.1.34 D-erythrulose 4-phosphate Brucella abortus 2308 the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues D-erythrose 4-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B58 Brucella sp.
-
-
-
2.7.1.215 Brucella abortus Q2YIQ1
-
-
2.7.1.215 Brucella abortus 2308 Q2YIQ1
-
-
5.1.3.38 Brucella abortus
-
-
-
5.1.3.38 Brucella abortus 2308
-
-
-
5.3.1.33 Brucella abortus Q2YIQ6
-
-
5.3.1.33 Brucella abortus 2308 Q2YIQ6
-
-
5.3.1.34 Brucella abortus
-
-
-
5.3.1.34 Brucella abortus 2308
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.38
-
 Brucella abortus
5.3.1.33
-
 Brucella abortus
5.3.1.34
-
 Brucella abortus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B58 L-erythritol 4-phosphate + FAD
-
 Brucella sp. L-3-tetrulose 4-phosphate + FADH2
-
 ?
1.1.1.B58 L-erythritol 4-phosphate + NAD+
-
 Brucella sp. L-3-tetrulose 4-phosphate + NADH + H+
-
 ?
2.7.1.215 ATP + erythritol
-
 Brucella abortus ADP + D-erythritol 4-phosphate i.e. L-erythritol 4-phosphate ?
2.7.1.215 ATP + erythritol
-
 Brucella abortus 2308 ADP + D-erythritol 4-phosphate i.e. L-erythritol 4-phosphate ?
5.1.3.38 D-erythrulose 1-phosphate
-
 Brucella abortus L-erythrulose 1-phosphate
-
 ?
5.1.3.38 D-erythrulose 1-phosphate the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues Brucella abortus L-erythrulose 1-phosphate
-
 ?
5.1.3.38 D-erythrulose 1-phosphate
-
 Brucella abortus 2308 L-erythrulose 1-phosphate
-
 ?
5.1.3.38 D-erythrulose 1-phosphate the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues Brucella abortus 2308 L-erythrulose 1-phosphate
-
 ?
5.3.1.33 L-erythrulose 1-phosphate
-
 Brucella abortus D-erythrulose 4-phosphate
-
 ?
5.3.1.33 L-erythrulose 1-phosphate the enzyme participates in erythritol catabolism Brucella abortus D-erythrulose 4-phosphate
-
 ?
5.3.1.33 L-erythrulose 1-phosphate
-
 Brucella abortus 2308 D-erythrulose 4-phosphate
-
 ?
5.3.1.33 L-erythrulose 1-phosphate the enzyme participates in erythritol catabolism Brucella abortus 2308 D-erythrulose 4-phosphate
-
 ?
5.3.1.34 D-erythrulose 4-phosphate
-
 Brucella abortus D-erythrose 4-phosphate
-
 r
5.3.1.34 D-erythrulose 4-phosphate the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues Brucella abortus D-erythrose 4-phosphate
-
 r
5.3.1.34 D-erythrulose 4-phosphate
-
 Brucella abortus 2308 D-erythrose 4-phosphate
-
 r
5.3.1.34 D-erythrulose 4-phosphate the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues Brucella abortus 2308 D-erythrose 4-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B58 eryB
-
 Brucella sp.
5.1.3.38 eryC
-
 Brucella abortus
5.3.1.33 D-3-tetrulose-4-phosphate isomerase
-
 Brucella abortus
5.3.1.33 eryH
-
 Brucella abortus
5.3.1.33 TpiA2
-
 Brucella abortus
5.3.1.34 eryI
-
 Brucella abortus
5.3.1.34 RpiB
-
 Brucella abortus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.38 30
-
 assay at Brucella abortus
5.3.1.33 30
-
 assay at Brucella abortus
5.3.1.34 30
-
 assay at Brucella abortus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.38 8
-
 assay at Brucella abortus
5.3.1.33 8
-
 assay at Brucella abortus
5.3.1.34 8
-
 assay at Brucella abortus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B58 FAD
-
 Brucella sp.
1.1.1.B58 NAD+
-
 Brucella sp.

General Information

EC Number General Information Comment Organism
5.1.3.38 metabolism the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues Brucella abortus
5.3.1.33 malfunction the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues Brucella abortus
5.3.1.34 metabolism the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues Brucella abortus