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Literature summary extracted from

  • Mordukhova, E.A.; Pan, J.G.
    Stabilization of homoserine-O-succinyltransferase (MetA) decreases the frequency of persisters in Escherichia coli under stressful conditions (2014), PLoS ONE, 9, e110504.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.46 additional information the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth. Overexpression of MetA induces the greatest number of persisters at 42°C, which is correlated to an increased level of aggregated MetA. Substitution of the native metA gene on the Escherichia coli K-12 WE chromosome by a mutant gene encoding the stabilized MetA leads to reduction in persisters at the elevated temperature and in the presence of acetate, as well as lower aggregation of the mutated MetA. Decreased persister formation at 42°C is confirmed also in wild-type Escherichia coli K-12 W3110 and a fast-growing WErph+ mutant harboring the stabilized MetA. The frequency of persister formation is correlated to the aggregation of the MetA, more than 150fold increase in the frequency of persisters at 42°C result from an increased level of aggregated proteins. Stabilized MetA reduces persister formation in the presence of acetate Escherichia coli

General Stability

EC Number General Stability Organism
2.3.1.46 inherently unstable and prone to aggregation, the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.46 succinyl-CoA + L-homoserine Escherichia coli
-
 CoA + O-succinyl-L-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.46 Escherichia coli P07623 gene metA
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.46 additional information the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.46 succinyl-CoA + L-homoserine
-
 Escherichia coli CoA + O-succinyl-L-homoserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.46 homoserine-O-succinyltransferase
-
 Escherichia coli
2.3.1.46 MetA
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.46 44
-
 complete aggregation of wild-tyype enzyyme at 44°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.46 succinyl-CoA
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.3.1.46 metabolism homoserine-o-succinyltransferase catalyzes the first unique step in the de novo methionine biosynthetic pathway Escherichia coli
2.3.1.46 additional information the enzyme is prone to aggregation under many stress conditions, resulting in a methionine limitation in Escherichia coli growth Escherichia coli