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Literature summary extracted from

  • Skryhan, K.; Cuesta-Seijo, J.A.; Nielsen, M.M.; Marri, L.; Mellor, S.B.; Glaring, M.A.; Jensen, P.E.; Palcic, M.M.; Blennow, A.
    The role of cysteine residues in redox regulation and protein stability of Arabidopsis thaliana starch synthase 1 (2015), PLoS ONE, 10, e0136997.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.21 reduced thioredoxin f1
-
 Arabidopsis thaliana
2.4.1.21 reduced thioredoxin m4
-
 Arabidopsis thaliana
2.4.1.21 thioredoxin
-
 Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.21 expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.21 C164S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C164S the mutant shows 50% activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C164S/C265S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C164S/C545S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C164S/C545S the double mutant with 13% of wild type activity has considerably decreased redox sensitivity as compared to wild type enzyme (30% vs 77%) Arabidopsis thaliana
2.4.1.21 C209S the mutant shows wild type activity Arabidopsis thaliana
2.4.1.21 C261S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C265S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C265S the mutant shows 37% activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C265S/C545S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C265S/C545S the mutant shows 8% activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C442S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C458S the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C533S the mutant shows wild type activity Arabidopsis thaliana
2.4.1.21 C545S the mutant shows 27% activity compared to the wild type enzyme Arabidopsis thaliana
2.4.1.21 C545S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.21 Cu2+
-
 Arabidopsis thaliana
2.4.1.21 oxidized thioredoxin f1
-
 Arabidopsis thaliana
2.4.1.21 oxidized thioredoxin m4
-
 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.21 Arabidopsis thaliana
-
-
-
2.4.1.21 Arabidopsis thaliana Q9FNF2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.21 His-Trap column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.21 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.21 ADP-alpha-D-glucose + glycogen 187% activity compared to maltotriose Arabidopsis thaliana ADP + ?
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltoheptaose 80% activity compared to maltotriose Arabidopsis thaliana ADP + maltooctaose
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltohexaose 90% activity compared to maltotriose Arabidopsis thaliana ADP + maltoheptaose
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltooctaose 79% activity compared to maltotriose Arabidopsis thaliana ADP + maltononaose
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltopentaose 116% activity compared to maltotriose Arabidopsis thaliana ADP + maltohexaose
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltose 20% activity compared to maltotriose Arabidopsis thaliana ADP + ?
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltose 20% activity compared to maltotriose Arabidopsis thaliana ADP + maltotriose
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltotetraose 103% activity compared to maltotriose Arabidopsis thaliana ADP + maltopentaose
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltotriose 100% activity Arabidopsis thaliana ADP + maltotetraose
-
 ?
2.4.1.21 additional information less than 1% activity with D-glucose Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.21 SS1
-
 Arabidopsis thaliana
2.4.1.21 starch synthase 1
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.4.1.21 Arabidopsis thaliana the enzyme is in the reduced and active form during the day with active photosynthesis up