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Literature summary extracted from

  • Li, C.; Wu, A.; Go, R.; Malouf, J.; Turner, M.; Malde, A.; Mark, A.; Gilbert, R.
    The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions (2015), PLoS ONE, 10, e0125507.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.18 gene Sbe2a, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.18 E513D site-directed mutagenesis, the mutant shows a much lower activity compared to wild-type enzyme mSBEIIa Zea mays
2.4.1.18 additional information construction of enzyme point mutants by site-directed mutagenesis to change the chain-length distribution CLD by changing activity of enzyme SBE. The enzyme mutants show no or only a slight change in degree of polymerization of branched glucans Zea mays
2.4.1.18 R363K site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme mSBEIIa Zea mays
2.4.1.18 R456K site-directed mutagenesis, the mutant shows similar activity compared to wild-type enzyme mSBEIIa Zea mays
2.4.1.18 S349F site-directed mutagenesis, creates an additional binding site for glucose, the mutant shows a much lower activity compared to wild-type enzyme mSBEIIa Zea mays
2.4.1.18 Y352F site-directed mutagenesis, , the mutant shows a much lower activity compared to wild-type enzyme mSBEIIa Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Zea mays O24421 isozyme SBEIIa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18 recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.18 additional information molecular weight distribution of starch, amylose and amylopectin, overview. SBE is the only enzyme that generates glucan branches, i.e. it is the only chain-stopping substance for branch growth, and as a consequence SBE has a significant effect on the final structure of the resulting starch Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.18 SBE
-
 Zea mays
2.4.1.18 SBEIIA
-
 Zea mays
2.4.1.18 starch branching enzyme
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.18 30
-
 assay at Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.18 7.4
-
 assay at Zea mays

General Information

EC Number General Information Comment Organism
2.4.1.18 additional information Tyr352, Glu513, and Ser349 are important for mSBEIIa activity while Arg456 is important for determining the position at which the linear glucan is cut, enzyme active site structure, molecular dynamics simulations and modeling, overview Zea mays
2.4.1.18 physiological function SBE is the only enzyme that generates glucan branches, i.e. it is the only chain-stopping substance for branch growth, and as a consequence SBE has a significant effect on the final structure of the resulting starch Zea mays