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Literature summary extracted from

  • Seger, M.; Gebril, S.; Tabilona, J.; Peel, A.; Sengupta-Gopalan, C.
    Impact of concurrent overexpression of cytosolic glutamine synthetase (GS1) and sucrose phosphate synthase (SPS) on growth and development in transgenic tobacco (2015), Planta, 241, 69-81.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.14 gene SPS1, recombinant expression driven by the CaMV35S promoter in leaves of Nicotiana tabacum via Agrobacterium tumefaciens strain GV3101 transfection method, coexpression with soybean glutamine synthetase Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.14 additional information production of transgenic tobacco plants with a cytosolic soybean glutamine synthetase (GS1) gene and a sucrose phosphate synthase (SPS) gene from maize, both driven by the CaMV35S promoter. The SPS enzyme activity in the SPS and SPS/GS1 co-transformants are the same under both nitrogen regimens, low and high nitrogen. The GS1/SPS transformants show a phenotype similar to the SPS transformants, phenotype overview Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.14 Mg2+ required Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-glucose + D-fructose 6-phosphate Zea mays
-
 UDP + sucrose 6F-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Zea mays P31927 gene SPS1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 recombinant enzyme partially from Nicotiana tabacum leaves Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 UDP-glucose + D-fructose 6-phosphate
-
 Zea mays UDP + sucrose 6F-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.14 SPS
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.14 30
-
 assay at Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.14 95
-
 purified enzyme, 10 min, inactivation Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.14 7.5
-
 assay at Zea mays

General Information

EC Number General Information Comment Organism
2.4.1.14 metabolism the enzyme plays a key role in carbon metabolism by catalyzing the synthesis of sucrose Zea mays