Literature summary extracted from

Lager, I.; Glab, B.; Eriksson, L.; Chen, G.; Banas, A.; Stymne, S.
Novel reactions in acyl editing of phosphatidylcholine by lysophosphatidylcholine transacylase (LPCT) and acyl-CoA:glycerophosphocholine acyltransferase (GPCAT) activities in microsomal preparations of plant tissues (2015), Planta, 241, 347-358.

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.3.1.23	microsome	-	Carthamus tinctorius	-	-
2.3.1.B36	membrane	-	Arabidopsis thaliana	16020	-
2.3.1.B36	membrane	-	Brassica napus	16020	-
2.3.1.B36	membrane	-	Ricinus communis	16020	-
2.3.1.B36	membrane	-	Carthamus tinctorius	16020	-
2.3.1.B36	membrane	-	Crambe hispanica subsp. abyssinica	16020	-
2.3.1.B36	membrane	-	Ulmus glabra	16020	-
2.3.1.B36	microsome	-	Arabidopsis thaliana	-	-
2.3.1.B36	microsome	-	Brassica napus	-	-
2.3.1.B36	microsome	-	Ricinus communis	-	-
2.3.1.B36	microsome	-	Carthamus tinctorius	-	-
2.3.1.B36	microsome	-	Crambe hispanica subsp. abyssinica	-	-
2.3.1.B36	microsome	-	Ulmus glabra	-	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
2.3.1.23	acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine	Carthamus tinctorius	-	CoA + 1,2-diacyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	Arabidopsis thaliana	-	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	Brassica napus	-	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	Ricinus communis	-	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	Carthamus tinctorius	-	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	Crambe hispanica subsp. abyssinica	-	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	Ulmus glabra	-	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.1.23	Carthamus tinctorius	-	-	-
2.3.1.B36	Arabidopsis thaliana	-	-	-
2.3.1.B36	Brassica napus	-	-	-
2.3.1.B36	Carthamus tinctorius	-	-	-
2.3.1.B36	Crambe hispanica subsp. abyssinica	-	-	-
2.3.1.B36	Ricinus communis	-	-	-
2.3.1.B36	Ulmus glabra	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.3.1.23	seed	-	Carthamus tinctorius	-
2.3.1.B36	leaf	-	Arabidopsis thaliana	-
2.3.1.B36	root	-	Arabidopsis thaliana	-
2.3.1.B36	seed	developing	Brassica napus	-
2.3.1.B36	seed	developing	Ricinus communis	-
2.3.1.B36	seed	developing	Carthamus tinctorius	-
2.3.1.B36	seed	developing	Crambe hispanica subsp. abyssinica	-
2.3.1.B36	seed	developing	Ulmus glabra	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.3.1.23	acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine	-	Carthamus tinctorius	CoA + 1,2-diacyl-sn-glycero-3-phosphocholine	-	?
2.3.1.23	linolenoyl-CoA + 1-linolenoyl-sn-glycero-3-phosphocholine	-	Carthamus tinctorius	CoA + 1,2-dilinolenoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.23	linoleoyl-CoA + 1-linoleoyl-sn-glycero-3-phosphocholine	-	Carthamus tinctorius	CoA + 1,2-dilinoleoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.23	oleoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine	-	Carthamus tinctorius	CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.23	palmitoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine	preferred substrate	Carthamus tinctorius	CoA + 1,2-dipalmitoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.23	stearoyl-CoA + 1-stearoyl-sn-glycero-3-phosphocholine	-	Carthamus tinctorius	CoA + 1,2-distearoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	-	Arabidopsis thaliana	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	-	Brassica napus	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	-	Ricinus communis	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	-	Carthamus tinctorius	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	-	Crambe hispanica subsp. abyssinica	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	acyl-CoA + glycerophosphocholine	-	Ulmus glabra	CoA + 1-acyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linolenoyl-CoA + glycerophosphocholine	best substrate	Arabidopsis thaliana	CoA + 1-linolenoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linolenoyl-CoA + glycerophosphocholine	best substrate	Brassica napus	CoA + 1-linolenoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linolenoyl-CoA + glycerophosphocholine	best substrate	Ricinus communis	CoA + 1-linolenoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linolenoyl-CoA + glycerophosphocholine	best substrate	Carthamus tinctorius	CoA + 1-linolenoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linolenoyl-CoA + glycerophosphocholine	best substrate	Crambe hispanica subsp. abyssinica	CoA + 1-linolenoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linolenoyl-CoA + glycerophosphocholine	best substrate	Ulmus glabra	CoA + 1-linolenoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linoleoyl-CoA + glycerophosphocholine	-	Arabidopsis thaliana	CoA + 1-linoleoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linoleoyl-CoA + glycerophosphocholine	-	Brassica napus	CoA + 1-linoleoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linoleoyl-CoA + glycerophosphocholine	-	Ricinus communis	CoA + 1-linoleoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linoleoyl-CoA + glycerophosphocholine	-	Carthamus tinctorius	CoA + 1-linoleoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linoleoyl-CoA + glycerophosphocholine	-	Crambe hispanica subsp. abyssinica	CoA + 1-linoleoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	linoleoyl-CoA + glycerophosphocholine	-	Ulmus glabra	CoA + 1-linoleoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	palmitoyl-CoA + glycerophosphocholine	radioactive assay with [14C]16:0-CoA	Arabidopsis thaliana	CoA + 1-palmitoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	palmitoyl-CoA + glycerophosphocholine	radioactive assay with [14C]16:0-CoA	Brassica napus	CoA + 1-palmitoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	palmitoyl-CoA + glycerophosphocholine	radioactive assay with [14C]16:0-CoA	Ricinus communis	CoA + 1-palmitoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	palmitoyl-CoA + glycerophosphocholine	radioactive assay with [14C]16:0-CoA	Carthamus tinctorius	CoA + 1-palmitoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	palmitoyl-CoA + glycerophosphocholine	radioactive assay with [14C]16:0-CoA	Crambe hispanica subsp. abyssinica	CoA + 1-palmitoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	palmitoyl-CoA + glycerophosphocholine	radioactive assay with [14C]16:0-CoA	Ulmus glabra	CoA + 1-palmitoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	stearoyl-CoA + glycerophosphocholine	-	Arabidopsis thaliana	CoA + 1-stearoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	stearoyl-CoA + glycerophosphocholine	-	Brassica napus	CoA + 1-stearoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	stearoyl-CoA + glycerophosphocholine	-	Ricinus communis	CoA + 1-stearoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	stearoyl-CoA + glycerophosphocholine	-	Carthamus tinctorius	CoA + 1-stearoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	stearoyl-CoA + glycerophosphocholine	-	Crambe hispanica subsp. abyssinica	CoA + 1-stearoyl-sn-glycero-3-phosphocholine	-	?
2.3.1.B36	stearoyl-CoA + glycerophosphocholine	-	Ulmus glabra	CoA + 1-stearoyl-sn-glycero-3-phosphocholine	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.3.1.23	acyl-CoA:glycerophosphocholine acyltransferase	-	Carthamus tinctorius
2.3.1.23	GPCAT	-	Carthamus tinctorius
2.3.1.B36	acyl-CoA:glycerophosphocholine acyltransferase	-	Arabidopsis thaliana
2.3.1.B36	acyl-CoA:glycerophosphocholine acyltransferase	-	Brassica napus
2.3.1.B36	acyl-CoA:glycerophosphocholine acyltransferase	-	Ricinus communis
2.3.1.B36	acyl-CoA:glycerophosphocholine acyltransferase	-	Crambe hispanica subsp. abyssinica
2.3.1.B36	GPCAT	-	Arabidopsis thaliana
2.3.1.B36	GPCAT	-	Brassica napus
2.3.1.B36	GPCAT	-	Ricinus communis
2.3.1.B36	GPCAT	-	Carthamus tinctorius
2.3.1.B36	GPCAT	-	Crambe hispanica subsp. abyssinica
2.3.1.B36	GPCAT	-	Ulmus glabra

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.3.1.B36	30	-	assay at	Arabidopsis thaliana
2.3.1.B36	30	-	assay at	Brassica napus
2.3.1.B36	30	-	assay at	Ricinus communis
2.3.1.B36	30	-	assay at	Carthamus tinctorius
2.3.1.B36	30	-	assay at	Crambe hispanica subsp. abyssinica
2.3.1.B36	30	-	assay at	Ulmus glabra

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.3.1.B36	7.2	-	assay at	Arabidopsis thaliana
2.3.1.B36	7.2	-	assay at	Brassica napus
2.3.1.B36	7.2	-	assay at	Ricinus communis
2.3.1.B36	7.2	-	assay at	Carthamus tinctorius
2.3.1.B36	7.2	-	assay at	Crambe hispanica subsp. abyssinica
2.3.1.B36	7.2	-	assay at	Ulmus glabra

General Information

EC Number	General Information	Comment	Organism
2.3.1.B36	metabolism	the enzyme provides a distinct route of resynthesising phosphatidylcholine via lysophosphatidylcholine after its deacylation. This route does not require the degradation of the glycerophosphocholine into free choline, the activation of choline to CDP-choline, nor the utilization of CDP-choline by the CDP-choline:diacylglycerol cholinephosphotransferase. GPCAT activity plays a ubiquitous role inplant lipid metabolism	Carthamus tinctorius
2.3.1.B36	metabolism	the enzyme provides a novel route of resynthesising phosphatidylcholine via lysophosphatidylcholine after its deacylation. This route does not require the degradation of the glycerophosphocholine into free choline, the activation of choline to CDP-choline, nor the utilization of CDP-choline by the CDP-choline:diacylglycerol cholinephosphotransferase. GPCAT activity plays a ubiquitous role inplant lipid metabolism	Arabidopsis thaliana
2.3.1.B36	metabolism	the enzyme provides a novel route of resynthesising phosphatidylcholine via lysophosphatidylcholine after its deacylation. This route does not require the degradation of the glycerophosphocholine into free choline, the activation of choline to CDP-choline, nor the utilization of CDP-choline by the CDP-choline:diacylglycerol cholinephosphotransferase. GPCAT activity plays a ubiquitous role inplant lipid metabolism	Brassica napus
2.3.1.B36	metabolism	the enzyme provides a novel route of resynthesising phosphatidylcholine via lysophosphatidylcholine after its deacylation. This route does not require the degradation of the glycerophosphocholine into free choline, the activation of choline to CDP-choline, nor the utilization of CDP-choline by the CDP-choline:diacylglycerol cholinephosphotransferase. GPCAT activity plays a ubiquitous role inplant lipid metabolism	Ricinus communis
2.3.1.B36	metabolism	the enzyme provides a novel route of resynthesising phosphatidylcholine via lysophosphatidylcholine after its deacylation. This route does not require the degradation of the glycerophosphocholine into free choline, the activation of choline to CDP-choline, nor the utilization of CDP-choline by the CDP-choline:diacylglycerol cholinephosphotransferase. GPCAT activity plays a ubiquitous role inplant lipid metabolism	Crambe hispanica subsp. abyssinica
2.3.1.B36	metabolism	the enzyme provides a novel route of resynthesising phosphatidylcholine via lysophosphatidylcholine after its deacylation. This route does not require the degradation of the glycerophosphocholine into free choline, the activation of choline to CDP-choline, nor the utilization of CDP-choline by the CDP-choline:diacylglycerol cholinephosphotransferase. GPCAT activity plays a ubiquitous role inplant lipid metabolism	Ulmus glabra
2.3.1.B36	additional information	GPCAT enzyme activity in microsomal membrane preparations from Arabidopsis thaliana roots and leaves occurs at much lower activity level compared to oil seed membranes, e.g. from safflower (Carthamus tinctorius), castor bean (Ricinus communis), elm (Ulmus glabra), and rape seed (Brassica napus)	Arabidopsis thaliana
2.3.1.B36	additional information	GPCAT enzyme activity in microsomal membrane preparations from Brassica napus oil seeds occurs at about 50% lower activity level compared to oil seed membranes from safflower (Carthamus tinctorius), castor bean (Ricinus communis), and elm (Ulmus glabra)	Brassica napus
2.3.1.B36	additional information	GPCAT enzyme activity in microsomal membrane preparations from Crambe abyssinica oil seeds occurs at much lower activity level compared to oil seed membranes from safflower (Carthamus tinctorius), castor bean (Ricinus communis), and elm (Ulmus glabra)	Crambe hispanica subsp. abyssinica
2.3.1.B36	physiological function	glycerophosphocholine:acyl-CoA acyltransferase (GPCAT) activity provides a distinct direct route of phosphatidylcholine resynthesis via lysophosphatidylcholine following its deacylation. GPCAT and not acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) is the limiting step in the formation of phosphatidylcholine	Brassica napus
2.3.1.B36	physiological function	glycerophosphocholine:acyl-CoA acyltransferase (GPCAT) activity provides a distinct direct route of phosphatidylcholine resynthesis via lysophosphatidylcholine following its deacylation. GPCAT and not acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), which is catalyzing the next step in the pathway, is the limiting step in the formation of phosphatidylcholine	Arabidopsis thaliana
2.3.1.B36	physiological function	glycerophosphocholine:acyl-CoA acyltransferase (GPCAT) activity provides a distinct direct route of phosphatidylcholine resynthesis via lysophosphatidylcholine following its deacylation. GPCAT and not acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), which is catalyzing the next step in the pathway, is the limiting step in the formation of phosphatidylcholine	Ricinus communis
2.3.1.B36	physiological function	glycerophosphocholine:acyl-CoA acyltransferase (GPCAT) activity provides a distinct direct route of phosphatidylcholine resynthesis via lysophosphatidylcholine following its deacylation. GPCAT and not acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), which is catalyzing the next step in the pathway, is the limiting step in the formation of phosphatidylcholine	Carthamus tinctorius
2.3.1.B36	physiological function	glycerophosphocholine:acyl-CoA acyltransferase (GPCAT) activity provides a distinct direct route of phosphatidylcholine resynthesis via lysophosphatidylcholine following its deacylation. GPCAT and not acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), which is catalyzing the next step in the pathway, is the limiting step in the formation of phosphatidylcholine	Crambe hispanica subsp. abyssinica
2.3.1.B36	physiological function	glycerophosphocholine:acyl-CoA acyltransferase (GPCAT) activity provides a distinct direct route of phosphatidylcholine resynthesis via lysophosphatidylcholine following its deacylation. GPCAT and not acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), which is catalyzing the next step in the pathway, is the limiting step in the formation of phosphatidylcholine	Ulmus glabra