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Literature summary extracted from

  • Liu, J.; Zhang, C.; Wei, C.; Liu, X.; Wang, M.; Yu, F.; Xie, Q.; Tu, J.
    The RING finger ubiquitin E3 ligase OsHTAS enhances heat tolerance by promoting H2O2-induced stomatal closure in rice (2016), Plant Physiol., 170, 429-443.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.27 expressed in Nicotiana benthamiana and Escherichia coli Rosetta (DE3) cells Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.27 cytoplasm
-
 Oryza sativa 5737
-
2.3.2.27 nucleus
-
 Oryza sativa 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.27 S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine Oryza sativa
-
 [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.27 Oryza sativa
-
 cultivar Nipponbare
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.27 leaf blade highest expression Oryza sativa
-
2.3.2.27 leaf sheath
-
 Oryza sativa
-
2.3.2.27 panicle
-
 Oryza sativa
-
2.3.2.27 root
-
 Oryza sativa
-
2.3.2.27 seedling
-
 Oryza sativa
-
2.3.2.27 shoot
-
 Oryza sativa
-
2.3.2.27 stem
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.27 S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine
-
 Oryza sativa [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.27 HTAS heat tolerance at seedling stage Oryza sativa
2.3.2.27 RING finger ubiquitin E3 ligase
-
 Oryza sativa

Expression

EC Number Organism Comment Expression
2.3.2.27 Oryza sativa the enzyme expression is induced strongly (22fold) by 0.1 mM abscisic acid after 12 h. The enzyme is slightly induced (2-4fold) by heat (45°C), cold (4°C), NaCl (250 mM), PEG (20% (w/v)), and H2O2 (100 mM) treatments up

General Information

EC Number General Information Comment Organism
2.3.2.27 physiological function the enzyme enhances heat tolerance by promoting H2O2-induced stomatal closure Oryza sativa