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Literature summary extracted from

  • Walker, A.M.; Hayes, R.P.; Youn, B.; Vermerris, W.; Sattler, S.E.; Kang, C.
    Elucidation of the structure and reaction mechanism of sorghum hydroxycinnamoyltransferase and its structural relationship to other coenzyme a-dependent transferases and synthases (2013), Plant Physiol., 162, 640-651.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.133
-
 Sorghum bicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.133 structures in its apo-form and ternary complex with shikimate and 4-coumaroyl-CoA, which is converted to its product during crystal soaking. Residues threonine36, serine38, tyrosine40, histidine162, arginine371, and threonine384 are involved in catalysis and specificity. Histidine162 and threonine36 play a role in the catalytic mechanism. Substrate binding should occur sequentially, with 4-coumaroyl-CoA binding prior to the acyl acceptor molecule. Comparison of the structure of sorghum HCT with the HCT involved in chlorogenic acid synthesis in Coffea canephora Sorghum bicolor

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.133 H162A complete loss of activity Sorghum bicolor
2.3.1.133 R371A complete loss of activity Sorghum bicolor
2.3.1.133 S38A 40% of wild-type activity Sorghum bicolor
2.3.1.133 T36A 6% of wild-type activity Sorghum bicolor
2.3.1.133 T384A 9% of wild-type activity Sorghum bicolor
2.3.1.133 Y40A 62% of wild-type activity Sorghum bicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.133 0.0631
-
 shikimate mutant Y40A, pH 6.5, temperature not specified in the publication Sorghum bicolor
2.3.1.133 0.153
-
 shikimate wild-type, pH 6.5, temperature not specified in the publication Sorghum bicolor
2.3.1.133 0.678
-
 shikimate mutant S38A, pH 6.5, temperature not specified in the publication Sorghum bicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.133 49000
-
 static light scattering Sorghum bicolor

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.133 Sorghum bicolor C5XXB7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.133 4-coumaroyl-CoA + quinate low activity towards quinate Sorghum bicolor CoA + 5-O-(4-coumaroyl)quinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate
-
 Sorghum bicolor CoA + 5-O-(4-coumaroyl)shikimate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.133 monomer 1 * 49000, calculated Sorghum bicolor