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Literature summary extracted from

  • Li, J.; Li, Z.; Li, C.; Gou, J.; Zhang, Y.
    Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata (2014), Plant Cell Rep., 33, 1173-1185.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.170 gene PlUGT1 or UGT88E12, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli and in Saccharomyces cerevisiae strain WAT11 Pueraria montana var. lobata

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.170 additional information Saccharomyces cerevisiae cells expressing PlUGT1 are not able to glycosylate daidzin and puerarin Pueraria montana var. lobata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.170 0.0204
-
 genistein pH 8.0, 30°C Pueraria montana var. lobata
2.4.1.170 0.0299
-
 daidzein pH 8.0, 30°C Pueraria montana var. lobata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.170 UDP-glucose + daidzein Pueraria montana var. lobata
-
 UDP + daidzein 7-O-beta-D-glucoside i.e. daidzin ?
2.4.1.170 UDP-glucose + formononetin Pueraria montana var. lobata
-
 UDP + formononetin 7-O-beta-D-glucoside i.e. ononin ?
2.4.1.170 UDP-glucose + genistein Pueraria montana var. lobata
-
 UDP + genistein 7-O-beta-D-glucoside i.e. genistin ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.170 Pueraria montana var. lobata
-
 collected from Langxi County, Anhui Province, China, gene PlUGT1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.170 recombinant enzyme from Escherichia coli Pueraria montana var. lobata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.170 cell suspension culture
-
 Pueraria montana var. lobata
-
2.4.1.170 additional information transcript abundance of gee PlUGT1 is correlated with the accumulation pattern of isoflavone glycosides such as daidzin in Pueraria lobata plants or in cell suspension culture Pueraria montana var. lobata
-
2.4.1.170 root high enzyme expression level Pueraria montana var. lobata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.170 additional information the enzyme is relatively specific for isoflavone aglycones, while flavonoid substrates are poorly accepted. No activity with cyanidin, luteolin, kaempferol, and quercetin, naringenin and apigenin are poor substrates, substrate specificity, overview Pueraria montana var. lobata ?
-
 ?
2.4.1.170 UDP-alpha-D-glucose + isoliquiritigenin
-
 Pueraria montana var. lobata UDP + isoliquiritigenin 7-O-beta-D-glucoside
-
 ?
2.4.1.170 UDP-glucose + daidzein
-
 Pueraria montana var. lobata UDP + daidzein 7-O-beta-D-glucoside i.e. daidzin ?
2.4.1.170 UDP-glucose + daidzein high activity Pueraria montana var. lobata UDP + daidzein 7-O-beta-D-glucoside i.e. daidzin ?
2.4.1.170 UDP-glucose + formononetin
-
 Pueraria montana var. lobata UDP + formononetin 7-O-beta-D-glucoside i.e. ononin ?
2.4.1.170 UDP-glucose + genistein
-
 Pueraria montana var. lobata UDP + genistein 7-O-beta-D-glucoside i.e. genistin ?
2.4.1.170 UDP-glucose + genistein best substrate Pueraria montana var. lobata UDP + genistein 7-O-beta-D-glucoside i.e. genistin ?
2.4.1.170 UDP-glucose + liquiritigenin very low activity Pueraria montana var. lobata UDP + liquiritigenin 7-O-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.170 PlUGT1
-
 Pueraria montana var. lobata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.170 30
-
 assay at Pueraria montana var. lobata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.170 1.1
-
 genistein pH 8.0, 30°C Pueraria montana var. lobata
2.4.1.170 1.1
-
 daidzein pH 8.0, 30°C Pueraria montana var. lobata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.170 8
-
 assay at Pueraria montana var. lobata

Expression

EC Number Organism Comment Expression
2.4.1.170 Pueraria montana var. lobata the enzyme is strongly induced by methyl jasmonate signal in cell suspension culture up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.170 37
-
 daidzein pH 8.0, 30°C Pueraria montana var. lobata
2.4.1.170 54
-
 genistein pH 8.0, 30°C Pueraria montana var. lobata