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Literature summary extracted from

  • Chormova, D.; Franková, L.; Defries, A.; Cutler, S.; Fry, S.
    Discovery of small molecule inhibitors of xyloglucan endotransglucosylase (XET) activity by high-throughput screening (2015), Phytochemistry, 117, 220-236.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.207 (-)-epicatechin gallate
-
 Petroselinum sp.
2.4.1.207 (-)-epigallocatechin gallate non-specific Petroselinum sp.
2.4.1.207 1-amino-4-hydroxy-2-methoxyanthracene-9,10-dione
-
 Petroselinum sp.
2.4.1.207 3-([3-(4-hydroxyphenyl)-3-oxopropanoyl]amino)-4-[methyl(octadecyl)amino]-benzoic acid
-
 Petroselinum sp.
2.4.1.207 4-([(4-methylphenyl)thio]methyl)-N-(2-pyridinylmethyl)benzamide
-
 Petroselinum sp.
2.4.1.207 4-chloromercuribenzoate
-
 Petroselinum sp.
2.4.1.207 6-bromo-2-hydroxy-1Hbenzo[de]isoquinoline-1,3-(2H)-dione
-
 Petroselinum sp.
2.4.1.207 aniline blue
-
 Petroselinum sp.
2.4.1.207 baicalein
-
 Petroselinum sp.
2.4.1.207 brilliant blue G specific inhibition Petroselinum sp.
2.4.1.207 Bromocresol green
-
 Petroselinum sp.
2.4.1.207 bromocresol purple
-
 Petroselinum sp.
2.4.1.207 ebselen
-
 Petroselinum sp.
2.4.1.207 eosin
-
 Petroselinum sp.
2.4.1.207 erythrosin
-
 Petroselinum sp.
2.4.1.207 hesperetin
-
 Petroselinum sp.
2.4.1.207 luteolin
-
 Petroselinum sp.
2.4.1.207 m-digallic acid
-
 Petroselinum sp.
2.4.1.207 additional information high-throughput fluorescent dot-blot screening to search for enzyme inhibitors in vitro, detailed overview. Of 4216 xenobiotics tested, with cellulose-bound xyloglucan as donor-substrate, 18 inhibit XET activity and 18 promote it (especially anthraquinones and flavonoids). No compounds promotes XET in quantitative assays with (cellulose-free) soluble xyloglucan as substrate, suggesting that promotion is dependent on enzyme-cellulose interactions. With cellulose-free xyloglucan as substrate, 22 XET-inhibitors are identified, especially compounds that generate singlet oxygen. Inhibitors include tannins, sulphydryl reagent,s and triphenylmethanes Petroselinum sp.
2.4.1.207 Mycophenolic acid
-
 Petroselinum sp.
2.4.1.207 N'-(1-E)-(5-bromofuran-2-yl)-methylidene-2-(3-methylphenoxy)-acetohydrazide
-
 Petroselinum sp.
2.4.1.207 Phenolphthalein
-
 Petroselinum sp.
2.4.1.207 phenylmercuric acetate
-
 Petroselinum sp.
2.4.1.207 phloretin
-
 Petroselinum sp.
2.4.1.207 retinoic acid
-
 Petroselinum sp.
2.4.1.207 riboflavin the riboflavin inhibitory effect is light-dependent, non-specific Petroselinum sp.
2.4.1.207 Tannic acid
-
 Petroselinum sp.
2.4.1.207 thiomersal
-
 Petroselinum sp.
2.4.1.207 vulpinic acid specific inhibition Petroselinum sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.207 cell wall
-
 Petroselinum sp. 5618
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Petroselinum sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 additional information usage of cellulose-bound tamarind seed xyloglucan as donor substrate and [1-3H]XXXGol as acceptor substrate Petroselinum sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.207 XET
-
 Petroselinum sp.
2.4.1.207 xyloglucan endotransglucosylase
-
 Petroselinum sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.207 20
-
 assay at Petroselinum sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.207 5.5
-
 assay at Petroselinum sp.

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.1.207 0.027
-
 pH 5.5, 20°C Petroselinum sp. eosin
2.4.1.207 0.029
-
 pH 5.5, 20°C Petroselinum sp. riboflavin
2.4.1.207 0.036
-
 pH 5.5, 20°C Petroselinum sp. erythrosin
2.4.1.207 0.082
-
 pH 5.5, 20°C Petroselinum sp. Tannic acid
2.4.1.207 0.15
-
 pH 5.5, 20°C Petroselinum sp. aniline blue
2.4.1.207 0.27
-
 pH 5.5, 20°C Petroselinum sp. Bromocresol green
2.4.1.207 0.31
-
 pH 5.5, 20°C Petroselinum sp. 4-chloromercuribenzoate
2.4.1.207 0.38
-
 pH 5.5, 20°C Petroselinum sp. (-)-epigallocatechin gallate
2.4.1.207 0.43
-
 pH 5.5, 20°C Petroselinum sp. (-)-epicatechin gallate
2.4.1.207 0.47
-
 pH 5.5, 20°C Petroselinum sp. m-digallic acid
2.4.1.207 0.52
-
 pH 5.5, 20°C Petroselinum sp. baicalein
2.4.1.207 0.52
-
 pH 5.5, 20°C Petroselinum sp. Phenolphthalein
2.4.1.207 0.58
-
 pH 5.5, 20°C Petroselinum sp. phloretin
2.4.1.207 0.61
-
 pH 5.5, 20°C Petroselinum sp. hesperetin
2.4.1.207 0.62
-
 pH 5.5, 20°C Petroselinum sp. vulpinic acid
2.4.1.207 0.7
-
 pH 5.5, 20°C Petroselinum sp. luteolin

General Information

EC Number General Information Comment Organism
2.4.1.207 evolution xyloglucan endotransglucosylase (XET) is a homotransglycanase enzyme activity found in all land plants and in some charophytic algae Petroselinum sp.