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Literature summary extracted from

  • Sarkar, T.R.; Battula, V.L.; Werden, S.J.; Vijay, G.V.; Ramirez-Pena, E.Q.; Taube, J.H.; Chang, J.T.; Miura, N.; Porter, W.; Sphyris, N.; Andreeff, M.; Mani, S.A.
    GD3 synthase regulates epithelial-mesenchymal transition and metastasis in breast cancer (2015), Oncogene, 34, 2958-2967.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.3.8 diagnostics enzyme expression predicts poor clinical outcome in breast cancer Homo sapiens
2.4.3.8 drug development the enzyme can serve as a potential druggable target for inhibiting tumor initiation and metastasis. GD3S expression correlates with activation of the c-Met signaling pathway leading to increased stem cell properties and metastatic competence, the GD3S-c-Met axis might serve as an effective target for the treatment of metastatic breast cancers Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.8 gene ST8SIA1, quantitative reverse-transcription PCR enzyme expression analysis Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.8 additional information analysis of the role of enzyme GD3S in primary tumor formation and metastasis in syngeneic wild-type BALB/c mice, in which tumors are developed, and in 4T1 cells, inhibiting or supressing the enzyme by triptolide-treatment and expressing control shRNA or GD3S shRNA, respectively. FOXC2 is silenced in MDA-MB-231 and HMLE-Snail cells. Phenotypes, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.8 triptolide
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.8 CMP-N-acetylneuraminate + ganglioside GM3 Homo sapiens
-
 CMP + ganglioside GD3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.8 Homo sapiens Q92185 gene ST8SIA1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.8 breast cancer cell
-
 Homo sapiens
-
2.4.3.8 MCF-10A cell
-
 Homo sapiens
-
2.4.3.8 MDA-MB-231 cell
-
 Homo sapiens
-
2.4.3.8 SUM-159PT cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.8 CMP-N-acetylneuraminate + ganglioside GM3
-
 Homo sapiens CMP + ganglioside GD3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.8 GD3 synthase
-
 Homo sapiens
2.4.3.8 GD3S
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.4.3.8 Homo sapiens transcription factor FOXC2, a central downstream effector of several epithelial-mesenchymal transition pathways, directly regulates GD3S expression by binding to its promoter additional information

General Information

EC Number General Information Comment Organism
2.4.3.8 malfunction inhibition of the enzyme using small hairpin RNA or triptolide compromises the initiation and maintenance of epithelial-mesenchymal transition instigated by various signaling pathways, including Snail, Twist and transforming growth factor-beta1 as well as the mesenchymal characteristics of claudin-low breast cancer cell lines, SUM159 and MDA-MB-231. Inhibition of enzyme GD3S in vivo prevents metastasis, it inhibits invasion and motility of cancer cells Homo sapiens
2.4.3.8 physiological function GD3 synthase is a critical enzyme involved in GD2 biosynthesis. The enzyme is necessary for wound healing, migration, invasion and stem cell properties in vitro. The enzyme has an important role in the initiation and maintenance of epithelial-mesenchymal transition. Transcription factor FOXC2, a central downstream effector of several epithelial-mesenchymal transition pathways, directly regulates GD3S expression by binding to its promoter. Enzyme GD3S expression correlates with activation of the c-Met signaling pathway leading to increased stem cell properties and metastatic competence Homo sapiens